中国实验动物学报2011，Vol.19Issue(4)：351-353,3.DOI:10.3969/j.issn.1005-4847.2011.04.017
腹腔一次注射法构建百草枯致小鼠肺间质纤维化模型
Establishment of a mouse model of pulmonary fibrosis induced by single intraperitoneal injection of paraquat
摘要
Abstract
Objective To develop an easy- and simple method of establishing mouse model of paraquat-induced pulmonary fihrosis. Methods Thirty-three C57 BL/6 J mice were divided randomly into the experimental group ( n = 30) and control group( n = 3 ). Paraquat ( 10 mg/kg) was administrated to the animals of the experimental group by a single in-traperitoneal injection. The mice were killed on the day 2,5,7, 14 and 28 after paraquat administration. The mice in the control group were treated by an intraperitoneal injection of physiological saline and killed on the 28th day. The general conditions, macroscopic structure and pathological changes of lung in the mice of both groups were examined and analyzed. Results The manifestation of intoxication was shown at 2 h after paraquat administration and obvious fibrosis of the lung tissue was observed on the day 28 in the experimental group. Conclusions A mouse model of pulmonary fibrosis can be established conveniently and reliably by a single intraperitoneal injection of paraquat. It may be used to study the pathogen-esis and treatment of paraquat-induced pulmonary fibrosis.关键词
肺间质纤维化/百草枯/中毒/动物模型Key words
Pulmonary fibrosis/ Paraquat/ Intoxication/ Animal model分类
生物科学引用本文复制引用
董雪松,刘盛业,刘伟,刘淑英,刘志..腹腔一次注射法构建百草枯致小鼠肺间质纤维化模型[J].中国实验动物学报,2011,19(4):351-353,3.基金项目
辽宁省教育厅课题(编号:2008785) （编号:2008785）
高等学校博士学科点专项科研基金课题(编号:20092104110001). （编号:20092104110001）
