中国实验动物学报2011，Vol.19Issue(4)：354-357,4.DOI:10.3969/j.issn.1005-4847.2011.04.018
转化生长因子-β1对大鼠骨髓间充质干细胞增殖及Slug表达的影响
Effects of transforming growth factor-β1 on rat bone marrow mesenchymal stem cell proliferation and Slug expression
钟伟 1王学文 1尹崇高 2贺良3
作者信息
- 1. 山东省潍坊医学院附属医院骨外科,山东潍坊 261041
- 2. 潍坊医学院护理学院,山东潍坊 261053
- 3. 北京积水潭医院创伤骨科,北京 100035
- 折叠
摘要
Abstract
Objective To detect the effect of transforming growth factor-β1 (TGF-β1) on cell proliferation and Slug expression of bone marrow mesenchymal stem cells (BMMSCs). Methods Density gradient centrifugation combined with adherence culture was used to isolate and culture rat BMMSCs. BMMSCs cultured to the 3rd passage were characterized by immunohistochemistry. The effects of TGF-β1 at different concentrations on cell proliferation were measured by MTT test. The expression of Slug protein was detected by immunofluorescence assay and Western blot. Results The rat BMMSCs were effectively isolated and purified by density gradient centrifugation combined with adherence culture. The results of immunohistochemical staining showed that the BMMSCs had positive expression of CD29 and CD44 while negative expression of CD34 and CD4S. TGF-β1 in low concentration showed promoting effect on cell proliferation, but TGF-β1 in high concentration inhibited cell proliferation of the rat BMMSCs. The expression of Slug protein increased after treatment with TGF-β1 for 24 h. Conclusions TGF-β1 at a certain concentration can promote BMMSCs proliferation and expression of Slug protein.关键词
转化生长因子-β1/骨髓间充质干细胞/增殖/SlugKey words
Transforming growth factor-β1/ Bone marrow mesenchymal stem cells/ Proliferation/ Slug分类
生物科学引用本文复制引用
钟伟,王学文,尹崇高,贺良..转化生长因子-β1对大鼠骨髓间充质干细胞增殖及Slug表达的影响[J].中国实验动物学报,2011,19(4):354-357,4.
