中国实验动物学报2011，Vol.19Issue(4)：358-360,S1-S2,5.DOI:10.3969/j.issn.1005-4847.2011.04.019
从ICR小鼠脂肪组织对resistin基因的扩增
PCR amplification of resistin gene from adipose tissue of ICR mice
摘要
Abstract
Objective To amplify the resistin gene from adipose tissue of ICR mice for further research of its functions. Methods Total RNA was extracted from the adipose tissue for amplification of resistin gene by RT-PCR. The sequence of amplified nucleotide acids was analyzed by DNAMAN software after gel electrophoresis and sequencing. Results The total RNA had good integrity. The expected 363 bp fragment was obtained by RT-PCR. The base peak was uniform in both directions of sequencing. The amplified fragment showed a 93. 8% homology with the reference of mouse resistin gene in GenBank, indicating that the resistin gene of ICR mice was obtained. Conclusion The resestin gene of ICR mice has been successfully amplified from adipod tissue, which laid a foundation for further studies on biological functions of resistin gene in ICR mice.关键词
ICR小鼠/脂肪组织/resistin基因/扩增Key words
ICR mice/ Adipose tissue/ Resistin gene/ Amplification分类
生物科学引用本文复制引用
鲁帅尧,赵远,唐东红,和占龙,禹文海,邵聪文..从ICR小鼠脂肪组织对resistin基因的扩增[J].中国实验动物学报,2011,19(4):358-360,S1-S2,5.基金项目
云南省应用基础研究面上项目(编号:2009ZC185M). （编号:2009ZC185M）
