中国科学院研究生院学报2011，Vol.28Issue(4)：551-555,5.
印度芥菜重金属ATP酶基因的分离与表达
Isolation and expression of heavy metal ATPase in Brassica juncea L.
摘要
Abstract
Brassica juncea L. Is a Zn/Cd tolerance plant, and the gene expression of heavy metal ATPase (HMA) has not been reported. In the present work, two Cdna fragments of BjHMA, BJHMA3 and BJHMA4, were isolated from B. Juncea seedling. Real-time quantitative PCR analysis revealed that both BJHMA3 and BJHMA4 were constitutively expressed in all tissue and preferentially in root. The Mrna profile of BJHMA3 or BjHMA4 was the lowest in leaf while the expressions were strongly enhanced by Zn or Cd stress, indicating that BjHMA played important roles in growth and in heavy metal homeostasis and tolerance in plant.关键词
印度芥菜/重金属ATP酶/基因表达Key words
Brassica juncea L./heavy metal ATPase/gene expression分类
生物学引用本文复制引用
张玉秀,张媛雅,柴团耀..印度芥菜重金属ATP酶基因的分离与表达[J].中国科学院研究生院学报,2011,28(4):551-555,5.基金项目
国家转基因生物新品种培育重大专项(2009ZX08009-130B)和中央高校基本科研业务费专项资金(2010YH05)资助 （2009ZX08009-130B）
