中医学报2011，Vol.26Issue(9)：1027-1030,4.
蜂毒素抑制骨肉瘤血管生成拟态与影响肿瘤细胞增殖、调亡的关系
Experimental Study of the Relation of Inhibiting Vasculogenic Mimicry and Inducing Cell Apoptosis of Melittin on Osteosarcoma Xenotransplanted Models of Nude Mice
摘要
Abstract
Objective:To study the antitumor effects and mechanism of Melittin on osteosarcoma xenotransplanted models of nude mice. Methods ; Xenotransplanted models of nude mice osteosarcoma cell UMR - 106 in the laevo - hind tibia of nude mice were established. Inoculated mice were randomly divided into normal saline group, positive control group, Melittin group. All the nude mice were sacrificed after treatment, the size and weight of tumor were measured and the tumor volumes and the inhibition rates of tumor were calculated, Expression of PCNA was deteced by immunohistochemical method. The VM density in tumor tissues was detected by CD34 and PAS double staining in vivo. TUNEL semi - quantitative assay was used to study the melittin - induced apoptosis in OS cell line. To study mainly the relation of inhibiting vasculogenic mimicry and inducing cell apoptosis. Results : Inhibiting rate ofmelittin in tumor capacity and weight was respectively 42. 98% ,39. 03%. The mice treated with Melittin groups showed significantly smaller volume and weight of tumor than those of NS group after treatment. The VM density and PCNA of the Melittin group were significantly lower than those of the NS group. After treated by melittin, the apoptotic index detected by TUNEL assay increased remarkably, the relation of inhibiting vasculogenic mimicry and inducing cell apoptosis of Melittin is positive correlation, the relation of inhibiting vasculogenic mimicry and cell multiplication of Melittin is negative correlation. Conclusion ;The mechanism of the inhibition of the implanted tumor growth with Melittin treatment may be associated with the inhibition of vasculogenic mimicry in transplanted tumor. Furthermore,Melittin can induce cell apoptosis and inhibit cell multiplication.关键词
蜂毒素/骨肉瘤/癌/血管生成拟态/裸鼠Key words
melittin/osteosarcoma/cancer/vasculogenic mimicry/nude mice分类
医药卫生引用本文复制引用
高启龙,姚亚民,杨峰,田同德,陈永强..蜂毒素抑制骨肉瘤血管生成拟态与影响肿瘤细胞增殖、调亡的关系[J].中医学报,2011,26(9):1027-1030,4.基金项目
国家自然科学基金(编号:30472223) （编号:30472223）
河南省基础与前沿项目(编号:092300410022) （编号:092300410022）
河南省医学科技攻关资助项目(编号:201001019) （编号:201001019）
