昆虫学报2011，Vol.54Issue(8)：869-876,8.
家蚕化学感受蛋白BmCSP4表达谱及结合特性分析
Expression pattern and binding specificity of chemosensory protein BmCSP4 in the silkworm, Bombyx mori
摘要
Abstract
Chemosensory proteins ( CSPs) are a class of small soluble proteins in insects, which are supposed to recognize and transport non-volatility odour molecules and chemical stimuli. In this study, we investigated the expression pattern of BmCSP4 using semi-quantitative RT-PCR. The results showed that BmCSP4 was expressed across various developmental stages, with gradual decrease from 4th larva to pupa. The tissue expression profile in males and females at adult stage was further studied, and the results showed that the expression level of BmCSP4 was lower in female head ( with antennae removed) , throax and abdomen than in other female tissues. The binding specificity of BmCSP4 to 15 compounds was tested using N-phenyl-1 -naphthylamine (1 -NPN ) as fluorescent probe, and the results showed that only benzaldehyde, p-methoxy benzaldehy, 2-pentyl-3-phenylpropenoic, 1-phenyl-l-butanol and acetophenone at the concentration of 10 junol/L replaced 1-NPN from BmCSP4 by 50% , with the dissociation constants of 3.20, 2.24, 2.88, 2.04 and 2. 52 jjimol/L, respectively. These results suggest that BmCSP4 may have diverse physiological function in different developmental stages, and may be involved in the process of discriminating aromatic aldehyde and aromatic ketone compounds.关键词
家蚕/化学感受蛋白/半定量RT-PCR/1-NPN/荧光结合Key words
Bombyx mori/ chemosensory protein/ semi-quantitative PCR/ N-phenyl-1-naphthylamine/fluorescence binding assay分类
生物科学引用本文复制引用
邓培渊,乔惠丽,李丹丹,鲁云风,李生才,阚云超..家蚕化学感受蛋白BmCSP4表达谱及结合特性分析[J].昆虫学报,2011,54(8):869-876,8.基金项目
国家自然科学基金项目(30700070) （30700070）
