昆虫学报2011，Vol.54Issue(8)：927-932,6.
昆虫RNA沉默抗病毒机制研究进展
Advances in the mechanism of antiviral RNA silencing in insects
摘要
Abstract
RNA silencing is identified as a general and evolutionarily conserved antiviral defense mechanism in insects. Meanwhile, viruses adapt the strategy by encoding suppressors of RNA silencing to counter the host-defense. Based on the related achievements in Drosophila, this article reviews the mechanism of antiviral RNA silencing, the characteristics and functions of RNA silencing suppressors, and the coevolution between hosts and viruses. Studies showed that RNA interference mediated by siRNA (small interfering RNAs, siRNA) plays a vital role in antiviral defense in Drosophila. Dicer-2(Dcr-2) , argonaute-2 (AG02) and R2D2 are three key components in siRNA pathway in Drosophila. Flies with these three genes knockdowned were hypersensitive to RNA virus infection. Additionally, several core genes involved in RNA interference were identified, including vasa intronic gene, aubergine, armitage, rm62 and piwi, which also play roles in antiviral defense in Drosophila. Three viral suppressors of RNAi (VSRs) have been identified in Drosophila viruses, I. E., FHV-B2, DCV-1A and CrPV-lA. FHV-B2 and DCV-1A inhibit RNA silencing by binding dsRNA or siRNA while CrPV-lA by targeting AGO2. During the boundless process of evolution, the hosts and viruses counter each other as well as coevolve. Components of antiviral silencing against highly diverse VSRs should be continuously and rapidly evolving.关键词
昆虫/RNA干扰/抗病毒机制/RNA沉默抑制子/共进化Key words
Insect/ RNA interference/ antiviral mechanism/ suppressors of RNA silencing/ coevolution分类
生物科学引用本文复制引用
吴萍,郭锡杰,周加春..昆虫RNA沉默抗病毒机制研究进展[J].昆虫学报,2011,54(8):927-932,6.基金项目
国家自然科学基金项目(30972143) （30972143）
江苏省自然科学基金项目(BK2010353) （BK2010353）
江苏科技大学引进人才科研启动项目(35181001) （35181001）
