南京林业大学学报（自然科学版）2011，Vol.35Issue(4)：1-6,6.DOI:10.3969/j.issn.1000-2006.2011.04.001
杨树接种溃疡病菌Botryosphaeria dothidea后蛋白质表达差异分析
Differential analysis of two-dimensional gel electrophoresis profiles of protein in poplars after inoculation with Botryosphaeria dothidea
摘要
Abstract
By two-dimension gel electrophoresis, differential proteomic analysis was conducted on Populus beijingensis (sensitive to Botryosphaeria dothidea) and P. Tomentosa (resist to B. Dothidea) before and after inoculated with B. Do-thidea (2-DE). Comparison of the 2-DE maps of proteins from the two inoculated and control species. The results showed that 35 3-fold different proteins were detected between the two species. Among which there were 4 up-regulated proteins, 15 down-regulated proteins before inoculation, and 7 up-regulated proteins, 9 down-regulated proteins after inoculation. For P. Tomentosa, there were 8 up-regulated proteins and 6 down-regulated proteins detected after inoculation, and for P. Beijingensis, 8 up-regulated proteins and 10 down-regulated proteins were identified after inoculation The analysis was taken on the produce conditions and contents of these proteins. It could be deduced that the up-regulated proteins expressed in P. Tomentosa before and after inoculation may be associated with consistutive resistance mechanisms and induced resistance mechanisms respectively. Four of them were detected by mass spectrometry, which were ribulose bisphosphate carboxylase small clain, predicted protein, superoxide dismutase, isopentenyl-diphosphate delta-isomerase respertively.These proteins may be associated with the resistance of poplar to canken.关键词
北京杨/毛白杨/溃疡病菌/双向凝胶电泳/差异蛋白质Key words
Populus beijingensis/P. Tomentosa/Botryosphaeria dothidea/two-dimensional gel electrophoresis/different proteins分类
农业科技引用本文复制引用
理永霞,吕全,梁军,严冬辉,贾秀贞,张星耀..杨树接种溃疡病菌Botryosphaeria dothidea后蛋白质表达差异分析[J].南京林业大学学报（自然科学版）,2011,35(4):1-6,6.基金项目
国家自然科学基金项目(30671682) （30671682）
科技基础性工作专项(2009FY210100) （2009FY210100）
