中药材2011，Vol.33Issue(7)：1020-1023,4.
壁虎醇提物对人食管癌EC-109细胞增殖抑制作用及其机制研究
Inhibitory Effects of Gecko Alcohol Extract on Human Esophageal Squamous Carcinoma Cell Line EC-109 Proliferation and Associated Mechanism
摘要
Abstract
Objective:To explore the proliferation inhibition effects of Gecko alcohol extract(GAE) on human esophageal squa-mous carcinoma cell line EC-109 and its mechanism. Methods; The inhibitory effects of GAE on proliferation of EC-109 cells were measured by MTT. Nucleolus change of apoptotic cells was observed by Hoechest33342 fluorescence staining. Apoptosis rate of EC-109 cells was detected by flow cytometry. The expressions of apoptosis protein Caspase-3 and FAS in EC-109 cells were investigated by im-munohistochemistry. Results: GAE had the inhibition effects on the proliferation of esophageal carcinoma cell EC-109. The apoptosis rate of EC-109 cell treated with GAE(3.0 mg/mL,4.0 mg/mL)for 48h was 20. 63% and 39. 73% .respectively. Compared with control group,the expression of Fas and Caspase-3 was significantly up-regulated in GAE treated group. Conclusion; GAE can inhibit the proliferation of esophageal carcinoma EC-109 cells and induce them apoptosis which may be correlated with increasing expression of protein Fas and Caspase-3.关键词
壁虎醇提物/细胞凋亡/食管癌/Caspase-3/FasKey words
Gecko alcohol extract/Apoptosis/Esophageal carcinoma/Caspase-3/Fas分类
医药卫生引用本文复制引用
宋佳玉,王晓兰,王建刚,席守民,刘玲,李瑞芳,余凯..壁虎醇提物对人食管癌EC-109细胞增殖抑制作用及其机制研究[J].中药材,2011,33(7):1020-1023,4.基金项目
河南省医学科技攻关计划项目(200903109) （200903109）
