中药材2011，Vol.33Issue(7)：1034-1037,4.
不同生长年限太白贝母中贝母辛和总生物碱的含量测定
Quantitative Determination of Peimisin and Total Alkaloids in Fritillaria taipaiensis of Different Growing Stage
摘要
Abstract
Objective; To study the content of peimisine and total alkaloids in Fritllaria taipaiensis of different growing stage. Methods: High performance liquid chromatography coupled with evaporative light scattering detector was employed,and peimisine was separated and quantified upon an Agilent Extend C18(250 mm × 4.0 mm,5μm),using acetonitrile-water-diethylamine as the mobile phase at the flow rate of 1.0 mL · min-1; UV spectrophotometry was used for the determination of total alkaloids. Results: During from two to three years period, the content of peimisine in F. Taipaiensis increased with growing stage, from four to six years, it decreased with growing stage;From two to four years,the content of total alkaloids in F. Taipaiensis increased with growing stage,from five to six years, it decreased with growing stage. Conclusion: It is suitable to harvest F. Taipaienssi of four years growing stage.关键词
太白贝母/贝母辛/总生物碱/高效液相色谱-蒸发光检测法/紫外分光光度法Key words
Fritillaria taipaiensis P. Y. Li/Peimisine/Total alkaloids/HPLC-ELSD/UV spectrophotometry分类
医药卫生引用本文复制引用
王怀玉,马鹏,彭锐..不同生长年限太白贝母中贝母辛和总生物碱的含量测定[J].中药材,2011,33(7):1034-1037,4.基金项目
重庆市卫生局资助项目(08-1-177) （08-1-177）
