中药材2011，Vol.33Issue(7)：1090-1093,4.
苦豆子总碱对SW480细胞株及裸鼠移植瘤的影响
Effect of Total Alkaloid of Sophora alopecuroides on SW480 Cells and Balb/c Nude Mice Tumor Xenograft
摘要
Abstract
Objective:To study the anti-tumor effect of total alkaloid of Sophora alopecvroides (TASA) on human colon adenocarci-noma SW480 cells and Balb/c nude mice tumor xenograft Methods;The effect of TASA on cell proliferation was assessed using MTT assay and the cell apoptosis was detected using Annexin V-FITC apoptosis assay. The nude mouse model bearing transplanted solid tumor SW480 was established. The changes of the volume and weight of the tumor were determined after treatment the mice with TASA. Fluorescence quantitative PCR was used to detect Caspase-3,Caspase-9 and BCL-2 mRNA expressions in the tumor. Results:TASA inhibited the proliferation of SW480 cells in a dose- and time-dependent manner. The apoptotic rate of cells was the best when the concentration of TASA was 0. 92 mg/mL at 48 hours. The volume and weight of the tumor xenograft in TASA groups were decreased when compared with those of the control group. The results of RT-PCR showed that TASA activated the pro-apoptotic Caspase-3 and Caspase-9 and lowered expressions of BCL-2. Conclusion;TASA can inhibit the growth of SW480 cells and the growth of transplanted solid tumor of human SW480 cell line,the mechanism of which involves the effect of Caspase-3, Caspase-9 and BCL-2 expression.关键词
苦豆子总碱/结肠癌/SW480细胞/裸鼠移植瘤/Caspase-3/Caspase-9/BCL-2Key words
Total alkaloid of Sophora alopecuroides/Inhibition/ SW480 cell/ Transplanted solid tumor/Caspase-3/Caspase-9/BCL-2分类
医药卫生引用本文复制引用
焦河玲,姚锐,邓虹珠,王晓娟,原海晓,姚晖..苦豆子总碱对SW480细胞株及裸鼠移植瘤的影响[J].中药材,2011,33(7):1090-1093,4.基金项目
广州市科技计划项目(2006Z2-E5053) （2006Z2-E5053）
