癌变·畸变·突变2011，Vol.23Issue(5)：325-329,5.DOI:10.3969/j.issn.1004-616x.2011.05.001
喹烯酮诱导HepG2细胞DNA复制相关基因表达水平的变化
Expression of DNA replication-related genes in HepG2 cells treated with quinocetone
摘要
Abstract
OBJECTIVE: Quinocetone induced S phase arrest, caused DNA and chromosome damage in HepG2 cells. Our purpose was to screen and analyse the mechanism of S phase arrest induced by Quinocetone. METHODS: The Agilent whole genome gene expression microarray was used to detect gene expression profile of HepG2 cells treated and untreated with Quinocetone. Futhermore, pathway analysis by Genespring software was carried out based on the differentially expressed genes, real time qPCR was used to verify some dramatically changed genes in DNA replication process. RESULTS: 1 750 differentially expressed genes, with 446 genes up-regulaled and 1 304 genes down-regulated, were found in HepG2 cells treated with Quinocetone in the total of 41 000 oligonudeotide fragments. Pathway analysis revealed that the differentially expressed genes gathered in functional pathways like cell cycle checkpoint, MAPK signaling pathway, DNA replication and DNA repair, etc. Moreover, the results of real lime qPCR focused on the changed DNA replication genes were consistent with cDNA microarray. CONCLUSION: The down-regulation of DNA replication genes caused incomplete DNA synthesis, and the DNA repair after its damage. These two events lead to S phase arrest after Quinocetone treatment. Moreover, the whole genome cDNA microarray analysis provided us much useful information for further research on the mechanism of 5 phase arrest induced by Quinocetone.关键词
喹烯酮/HepG2细胞/S期阻滞/表达谱基因芯片Key words
quinocetone/ HepG2 cells/ S phase arrest/ cDNA microarray分类
医药卫生引用本文复制引用
刘凤英,靳溪,汤树生,陈倩,张婷,赵文霞,张朝明,张燊,肖希龙..喹烯酮诱导HepG2细胞DNA复制相关基因表达水平的变化[J].癌变·畸变·突变,2011,23(5):325-329,5.基金项目
教育部博士点新教师基金(20100008120003) （20100008120003）
