作物学报2011，Vol.37Issue(7)：1205-1211,7.DOI:10.3724/SP.J.1006.2011.01205
大豆硬脂酸-ACP脱饱和酶基因启动子的克隆及其表达活性分析
Cloning and Activity Analysis of Soybean SACPD-C Promoter
摘要
Abstract
The S ACPD-Cp promoter of soybean S ACPD-C was isolated from the genomic DN A of soybean cultivar Jidou 2 using TAIL PCR. Promoter sequence analysis by PLACE showed that the cloned fragment contained many motifs that constituted the seed-specific cw-elements. Replacing CaMV35S promoter of pCAMBIA1301 with the SACPD-Cp fragment, the binary expression vector pCAM-SACPD-Cp was constructed. Transient expression by Agrobacterium tumefaciens mediated method, the his-tochemical GUS analysis and fluorometric GUS analysis were used for testing the expression of the GUS activity. The results indicated that GUS activity driven by SACPD-Cp fragment was 93.01% of that driven by CaMV35S promoter. The SACPD-Cp promoter did not have the homology compared with the reported promoters. GUS activity assays indicated that GUS was expressed only in seeds, but not in roots, stems and leaves, which suggests the SACPD-Cp is a seed-specific promoter.关键词
大豆/硬脂酸-ACP脱饱和酶基因/序列分析/种子特异性启动子/瞬时表达Key words
Soybean/ SACPD-C gene/ Sequence analysis/ Seed-specific promoter/ Transient expression引用本文复制引用
张庆林 ,赵艳,李晓薇 ,翟莹 ,张艳 ,王英 ,李景文,王庆钰..大豆硬脂酸-ACP脱饱和酶基因启动子的克隆及其表达活性分析[J].作物学报,2011,37(7):1205-1211,7.基金项目
本研究由国家转基因生物新品种培育重大专项(2008ZX08004-003),国家自然科学基金项目(30971808),吉林省科技发展计划重点项目(20080204),长春市科技局国际科技合作项目(08GH10)和“211”三期建设项目资助. （2008ZX08004-003）
