生命科学研究2011，Vol.15Issue(4)：317-322,6.
靶向抑制miRNA-21提高白血病K562细胞对阿糖胞苷的敏感性
Enhanced Chemosensitivty of Leukemic Cells to Cytarabine by Targeted Suppression of miRNA-21
摘要
Abstract
Our goal is to investigate the influences of anti-miRNA-21 oligonucleotide (AMO-miR-21) on Ara-C chemosensitivity in human leukemic K562 cells,which were incubated with different concentration of Ara-C alone or in combination with AMO-miR-21.The growth-inhibitory potencies were measured by MTT assay.The inhibition rate and IC50 was calculated.The cell apoptosis was assessed by flow cytometry.The expression of miRNA-21 and its potential target gene PdcdA was detected by real-time PCR and Western blot in K562 cells.Transfection of K562 cells with AMO-miR-21 enhanced the chemosensitivity of Ara-C by up to2.3-fold and promoted Ara-C-induced cell apoptosis.Suppressing the cellular levels of miRNA-21 significantly upregulated the expression of PdcdA.The results support a substantial role for AMO-miR-21 that enhance Ara-C sensitivity in K562 cells.The mechanism appears to be related to anti-miRNA-21-induced apoptosis,suggesting a novel potential approach to treatment of leukemia.关键词
miRNA-21/反义寡核苷酸/阿糖胞苷/K562细胞/化疗敏感性Key words
miRNA-21/antisense oligonucleotide/Ara-C/leukemia K562 cell/chemosensitivity分类
医药卫生引用本文复制引用
朱雪姣,李育敏,谷景义,郭敏,费嘉..靶向抑制miRNA-21提高白血病K562细胞对阿糖胞苷的敏感性[J].生命科学研究,2011,15(4):317-322,6.基金项目
暨南大学科研培育与创新基金前瞻性与基础研究项目(11609406) （11609406）
暨南大学研究生精品课程(医学分子生物学)建设项目(50623021) （医学分子生物学）
