生命科学研究2011，Vol.15Issue(4)：345-350,6.
Sonic hedgehog在二乙基亚硝胺诱发大鼠肝癌发生过程中的表达及意义
Expression and Significances of Sonic Hedgehog During Hepatocarcinogenesis Induced by Diethylnitrosamine in the Rats
摘要
Abstract
To observe expression of sonic hedgehog (Shh) in tissue of liver,to explore its role during hepato-carcinogenesis induced by diethylnitrosamine (DEN) in the rats.On the Basis of this liver cancer model,the morphological changes of liver tissue were observed by HE staining,the expression of Shh protein and mR-NA were measured with immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) respectively.Based on morphological characters during the process of hepatocarcinogenesis,the rats were divided into control group,hepatic lesion group,hepatic proliferation/cirrhosis group and hepatic carcinogene-sis group.The cells of Shh protein expression mainly distributed in the epithelia of interlobular bile duct,hy-perplasia node,cancerous tissues and pericancerous tissues.The positive expression rate of Shh protein inthe control group,hepatic toxic lesion,hepatic proliferation/cirrhosis and hepatic carcinogenesis were 0%,30%,53.33% and 79.17% (χ2=42.67,P<0.05) respectively.The expression rate of Shh mRNA increased gradually with the hepatocarcinogenesis (χ2=13.35,P<0.05).The result that the binds of Shh mRNA positive expression were analyzed showed that its expression increased gradually with the hepatocarcinogenesis (F= 110.26,P<0.05).The expression rate of Ptch mRNA increased gradually with the hepatocarcinogenesis(χ2=19.83,P<0.05).The result that the binds of Ptch mRNA positive expression were analyzed showed that its expression increased gradually with the hepatocarcinogenesis (F=68.28,P<0.05).Experimental results in-di-cated that the abnormal expression of Shh caused abnormal activation of Shh signaling pathway,and con-tin-ually affected the cells of liver,and involved the process of hepatocarcinogenesis.关键词
肝癌/Sonic hedgehog(Shh)/Ptch/二乙基亚硝胺(DEN)Key words
liver neoplasms/Sonic hedgehog(Shh)/Ptch/diethylnitrosamine(DEN)分类
医药卫生引用本文复制引用
王东,李红星,李笑岩,白咸勇,李雅娜..Sonic hedgehog在二乙基亚硝胺诱发大鼠肝癌发生过程中的表达及意义[J].生命科学研究,2011,15(4):345-350,6.基金项目
山东省自然科学基金资助项目(Y2008C95) （Y2008C95）
山东省高等学校科技计划项目(J09LF12) （J09LF12）
