中国药房2011，Vol.22Issue(29)：2708-2710,3.
家兔耳轻度冻伤模型构建中浸冻时间的筛选研究
Selection of Freezing Duration in the Establishment of Slight Frostbite Model of Rabbit Ear
摘要
Abstract
OBJECTIVE: To explore the freezing duration of slight frostbite model of rabbit ear. METHODS: Rabbis were randomly divided into normal control group, 20 s freezing group and 30 s freezing group with 6 rabbits in each group. Rabbit ears were soaked in 95% alcoholic balneum frigidum at -24.5 ℃ for different time (20 s and 30 s), then the appearance of rabbit ears, histopathology, swelling and distension degree of them in 8 d were observed. RESULTS: After freezing for 20 s, the skin of rabbit ear changed into small blister 12-48 h after freezing, and the injured site turned into normal 8 d after freezing; the thickening of skin could be observed and appendant of skin and cartilaginous tissue had no obvious change. After freezing for 30 s, the rabbit ear changed into serous blister 12-48 h after freezing and the injured site appeared edema after 8 d and the skin began to shedding; epidermis diabrosis and lesion could be observed under microscope and appendant of skin and cartilaginous tissue surround were infiltrated with inflammatory cells. Since 3 d after freezing, the swelling and distension degree of rabbit ears in 30 s freezing group was significantly higher than in 20 s freezing group (P<0.05). CONCLUSION: Slight frostbite model of rabbit ear can be established by freezing them in 95% alcoholic balneum frigidum at -24.5 ℃ for 20 s.关键词
兔耳/轻度冻伤/浸冻时间/模型构建Key words
Rabbit ear/Slight frostbite/Freezing duration/Model construction分类
医药卫生引用本文复制引用
韩晋,周旭,吕俊兰,陈红鸽,袁海龙..家兔耳轻度冻伤模型构建中浸冻时间的筛选研究[J].中国药房,2011,22(29):2708-2710,3.基金项目
国家“十一五”重大新药创制专项(军口)基金资助课题(2008ZXJ09004-014) （军口）
