中国中西医结合杂志2011，Vol.31Issue(9)：1224-1228,5.
姜黄素对单侧输尿管梗阻大鼠肾小管上皮细胞转分化及TGF-β/Smads信号转导途径的影响
Effects of Curcumin on the Epithelial Mesenchymal Transition and TGF-β/Smads Signaling Pathway in Unilateral Ureteral Obstruction Rats
摘要
Abstract
Objective To observe the effects of curcumin on the epithelial mesenchymal transition (EMT) and TGF-p/Smads signal pathway in unilateral ureteral obstruction (UUO) rats. Methods UUO rat model was established. Rats were divided into the sham-operation group, the model group, the 1st curcumin group, and the 2nd curcumin group, respectively. From the 2nd day after surgery, rats of the 1st curcumin group were administrated with curcumin by gastrogavage at the daily dose of 100 mg/kg. From the 10th day after surgery, rats of the 2nd curcumin group were administrated with curcumin by gastrogavage at the daily dose of 100 mg/kg. Equal volume of normal saline was given to rats by gastrogavage in the sham-operation group and the model group. On the 28th day after surgery rats were killed. The renal tissues of the obstructed side were taken out. The distribution of mice α-smooth muscle actin (α-SMA) and E-cadherin expressions were observed using immunohistochemical method. Transforming growth factor-β1(TGF-β1), P-Smad2, P-Smad3, and Smad7 expressions were detected by Western blot. TGF-β1, transforming growth factor-β receptor I (TβRI), collagen-Ⅰ (Col-Ⅰ), and collagen-Ⅲ (Col-Ⅲ) mRNA expressions were detected by Real-time fluorescent quantitative PCR. Results α-SMA expressions were obviously enhanced, E-cadherin expressions obviously weakened, the protein expression levels of P-Smad2 and P-Smad3, protein and mRNA expression levels of TGF-β1, TβRI mRNA, Col-Ⅰ mRNA, and Col-Ⅲ mRNA obviously up-regulated, Smad7 protein expressions obviously decreased (all P<0.01) in the renal tissues of rats in the UUO group. In the 1st curcumin group and the 2nd curcumin group, the protein expressions of α-SMA, P-Smad2, P-Smad3, and TGF-β1; mRNA expression levels of TGF-β1, TβRI mRNA, Col-Ⅰ, and Col-Ⅲ obviously decreased (P<0.05,P< 0.01), while the protein expressions of E-cadherin and Smad7 obviously increased (P < 0.05, P < 0.01). Conclusions Curcumin could intervene several sites of the TGF-β/Smads signal transduction pathway of UUO rats, inhibit the occurrence of EMT of renal tubular epithelial cells, thus attenuating the tubulo-interstitial fibrosis.关键词
单侧输尿管梗阻/肾小管上皮细胞转分化/姜黄素Key words
unilateral ureteral obstruction/ epithelial mesenchymal transition/ curcumin引用本文复制引用
李彧,陈朝青,李亚东,李健,牛建昭..姜黄素对单侧输尿管梗阻大鼠肾小管上皮细胞转分化及TGF-β/Smads信号转导途径的影响[J].中国中西医结合杂志,2011,31(9):1224-1228,5.基金项目
国家自然科学基金资助项目(No.30600819) （No.30600819）
