中国中西医结合杂志2011，Vol.31Issue(9)：1248-1253,6.
淫羊藿苷对哮喘小鼠肺内嗜酸性粒细胞凋亡和Bcl-2、Bax基因蛋白表达的影响
Effects of Icariin on Bcl-2 and Bax Protein Expressions and Eosinophils Apoptosis in Bronchial Asthmatic Mice
摘要
Abstract
Objective To study the effects of icarrin on Bcl-2 and Bax protein expressions and eosinophils apoptosis in bronchial asthmatic mice. Methods 48 female Balb/c mice were randomly divided into 6 groups, i. e., the normal control group, the model group, the Dexamethasone group, the low dose icariin group, the middle dose icariin group, and the high dose icariin group, 8 mice in each group. Bronchial asthma in mice were induced by irv traperitioneal sensitization and challenged with nebulized ovalbumin (OVA). The mice of each treatment group were administrated with different doses of icariin by peritoneal injection from the first asthma sensitization (the 3rd week after the modeling) to the day before killing once every other day, while mice in the normal control group were administrated with physiological saline. The mice were killed after 6 weeks of treatment. The apoptosis of eosinophils and the Bcl-2 and Bax protein expressions of the lung tissues were detected by TUNEL and immunohistochem-ical assay respectively. Results As compared with the model group, the apoptosis ratio of eosinophils were higher in the rest four treatment groups (P<0.05). The Bcl-2 protein positive areas in the lung tissues and the airway wall were significantly lowered (P<0.05). The Bax protein positive area significantly increased (P<0.05). Conclusion In bronchial asthmatic mice, icariin could enhance the apoptosis of eosinophils and lessen their infiltration by decreasing the expression of Bcl-2 protein and increasing the expression of Bax protein in lung.关键词
支气管哮喘/淫羊藿苷/嗜酸性粒细胞/细胞凋亡/Bcl-2和BaxKey words
bronchial asthma/ icariin/ eosinophil/ apoptosis/ Bcl-2 and Bax引用本文复制引用
杜文静,董竞成,蔡萃,张红英,孙婧,吕玉宝,李璐璐,徐长青..淫羊藿苷对哮喘小鼠肺内嗜酸性粒细胞凋亡和Bcl-2、Bax基因蛋白表达的影响[J].中国中西医结合杂志,2011,31(9):1248-1253,6.基金项目
国家重点基础研究发展计划973计划项目(No.2009CB523001) （No.2009CB523001）
上海市卫生局中医药科研基金项目(No.2008L018A) （No.2008L018A）
2009年度上海市优秀学科带头人计划(A类)项目(No.09XD1400700) （A类）
新教师基金课题(No.200802461053) （No.200802461053）
