中国医科大学学报2011，Vol.40Issue(8)：699-702,4.DOI:21-1227/R.20110726.1659.028
谷氨酰胺、地塞米松对脓毒症幼年大鼠小肠肿瘤坏死因子α与基质金属蛋白酶3的影响
Effects of Glutamine and Dexamethasone on Tumor Necrosis Factor Alpha and Matrix Metalloproteinase-3 of Small Intestine in Young Rat with Sepsis
摘要
Abstract
Objective To observe the dynamic changes of tumor necrosis factor alpha ( TNF-a ) and matrix metalloproteinase-3 ( MMP-3 ) of small intestine in young rats with sepsis and to investigate the effects of dexamethasone (Dex) and glutamine (Gln) on them. Methods 128 Wistar rats with 18 days of age were intraperitoneally injected with normal saline(8 rats),IPS(40 rats,4 mg/kg,Escherichia coli 055: B5),Dex (40 rats,LPS 4 mg/kg 1 hour late+ Dex 5 mg/kg) and Gln (40 rats,LPS 4 mg/kg + Gln 1 ml/kg),respectively. They were then sacrificed after 0,2,4,6,24 and 72 hours, and distal segments of ileum were collected, paraffin-embedded and fixed to measure the expressions of TNF-α and MMP-3 by immunohistochemistry. Results 1.Expression of TNF-α in LPS group gradually increased with the progression of the disease and reached the peak at 24 h,and was significantly higher versus control group at each time point (P < 0.01). In Dex group,this tend was less obvious,but expression of TNF-α at each time point was still significantly higher (P < 0.01). Expression of TNF-α was significant higher in Gin group versus IPS group at 6 h (P < 0.05). 2. Similar to TNF-α,expression of MMP-3 in LPS group also gradually increased with the progression of sepsis and reached the peak at 24 h, and was significantly higher than that in control group (P < 0.01 except at 6 h). However,expression of MMP-3 in Gln group was significantly lower (P < 0.01 except at 6 h). Expression of MMP-3 in Dex group was higher than Gln group but lower versus IPS group,and was significantly higher versus control group at each time point (P < 0.01). Conclusion 1. The inhibiting effect of Dex on inflammation was stronger than that of Gln. 2. Both Dex and Gin had inhibiting effects on MMP-3 synthesis induced by IPS,but effect of Glu was stronger.关键词
脓毒症/肿瘤坏死因子α/基质金属蛋白酶3/地塞米松/谷氨酰胺Key words
sepsis/ dexamethasone/ glutamine/ matrix metalloproteinase-3/ tumor necrosis factor-α分类
医药卫生引用本文复制引用
王虹,刘石,孙梅..谷氨酰胺、地塞米松对脓毒症幼年大鼠小肠肿瘤坏死因子α与基质金属蛋白酶3的影响[J].中国医科大学学报,2011,40(8):699-702,4.基金项目
辽宁省科技厅资助项目(20052091),沈阳市科技局资助项目(1063230-3-00) （20052091）
