南方医科大学学报2011，Vol.31Issue(8)：1411-1415,5.
载银珊瑚羟基磷灰石的载银量及其细胞相容性的实验研究
Determination of silver content in silver-loaded coral hydroxyapatite in relation to the biocompatibility
摘要
Abstract
Objective To detern.Lie the amount of silver in silver-loaded coral hydroxyapatite (Ag+-CHA) bone substitute and its impact on the biocompatibility of this material with mouse embryonic osteoblast cells. Methods Ag*-CHA was prepared by immersing coral hydroxyapatite in a serial concentration of silver nitrate solutions. The amount of silver in the prepared Ag+-CHA was measured by inductively coupled plasma atomic emission spectrometry (ICP-AES). The viability of MC3T3-E1 cells incubated with Ag+-CHA was measured by MTT colorimetric assay, and the cell growth and morphological changes were observed by inverted phase-contrast microscope and confocal laser scanning microscope. Results The amount of silver loading in the bone substitutes prepared by immersion in l×l02, l×l0-3, 5×10-4, 10-4, 8×l0-5, and 5×l0-5 mol/L silver nitrate solutions were 4127.67±47.35,167.90±ll.00, 83.42±4.51, 30.20±2.32, 22.39±4.09, and 15.11 ±0.55 μg/g, respectively. A low silver content in the material (prepared with silver nitrate solution of less than 8 x 105 mol/L) showed no significant inhibitory effect on the growth of MC3T3-E1 cells or produced noticeable cytotoxic effect. On the materials prepared with 8×l0-5 and 10-5 mol/L silver nitrate solution, the osteoblasts displayed active proliferation similar to those incubated on materials without silver loading. The confluent cells showed a normal fusiform morphology with tight arrangement. Conclusion Ag+-CHA with low silver content has a good biocompability and can promote the proliferation and growth of MC3T3-E1 cells in vitro, suggesting the clinical potential of this material as a anti-infection bone substitute.关键词
珊瑚羟基磷灰石/生物相容性/MTT比色法/抗感染骨替代材料/银Key words
coral hydroxyapatite/ biocompatibility/ anti-infection bone substitute/ silver分类
医药卫生引用本文复制引用
张宇,尹庆水,张余,李剑,陈旭琼,傅栋..载银珊瑚羟基磷灰石的载银量及其细胞相容性的实验研究[J].南方医科大学学报,2011,31(8):1411-1415,5.基金项目
军队“十一五”科技计划项目(06G045) （06G045）
广东省科技攻关项目(2003C 104004,2004B33101005) （2003C 104004,2004B33101005）
