南方医科大学学报2011，Vol.31Issue(8)：1440-1442,3.
山奈酚诱导人食管鳞癌Eca-109细胞凋亡及其机制
Kaempferol-induced apoptosis of human esophageal squamous carcinoma Eca-109 cells and the mechanism
摘要
Abstract
Objective To evaluate the growth-inhibiting and pro-apoptotic effect of kaempferol in human esophageal squamous carcinoma Eca-109 cells and explore the mechanism. Method The effect of kaempferol on Eca-109 cell proliferation in vitro was measured by MTT assay. TUNEL staining was used to detect the cell apoptosis following kaempferol treatment. The changes in Bax and Bcl-2 mRNA expressions in response to kaempferol treatment were determined by RT-PCR, and the caspase-3 and caspase-9 activities were evaluated using colorimetric assay. Results Kaempferol significantly inhibited Eca-109 cell proliferation (P<0.05) in a concentration-dependent manner and induced obvious cell apoptosis. RT-PCR showed that after kaempferol treatment caused up-regulated Bax and down-regulated Bcl-2 mRNA expression. The colorimetric assay revealed significantly increased caspase-3 and caspase-9 activities in Eca-109 cells following kaempferol treatment (P<0.01). Conclusion Kaempferol can induce apoptosis of Eca-109 cells via a mitochondria-dependent pathway.关键词
山奈酚/食管癌/细胞凋亡/Eca-109细胞株Key words
kaempferol/ esophageal carcinoma/ apoptosis/ Eca-109 cell line分类
医药卫生引用本文复制引用
李瑞君,梅家转,刘桂举..山奈酚诱导人食管鳞癌Eca-109细胞凋亡及其机制[J].南方医科大学学报,2011,31(8):1440-1442,3.基金项目
郑州市博士创业基金(郑卫2009175) （郑卫2009175）
