动物学研究2011，Vol.32Issue(4)：371-378,8.DOI:10.3724/SP.J.1141.2011.04371
非洲爪蟾中一种长型肽聚糖识别蛋白的克隆与表达分析
Cloning and expression analysis of a long type peptidoglycan recognition protein (PGRP-L) from Xenopus tropicalis
摘要
Abstract
Peptidoglycan recognition proteins (PGRPs) are a family of pattern recognition receptors (PRRs) of the immune system,which bind and hydrolyze bacterial peptidoglycan.Here,a long type PGRP (PGRP-L) was first cloned in the lower vertebrate species Xenopus tropicalis (Xt).The XtPGRP-L possessed a conserved genomic structure with five exons and four introns.The alignment and phylogenetic analysis indicated that XtPGRP-L might be a type of amidase-like PGRP.The 3-D model showed that XtPGRP-L possessed a conserved structure compared with the Drosophila PGRP-Lb.During embryonic development,XtPGRP-L was not expressed until the 72 h tadpole stage.In adult tissues,it was strongly expressed in the liver,lung,intestine,and stomach.Furthermore,after LPS stimulation,the expression of XtPGRP-L was up-regulated significantly in the liver,intestine and spleen,indicating that XtPGRP-L may play an important role in the innate immunity of Xenopus tropicalis.关键词
肽聚糖识别蛋白/基因克隆/表达分析/非洲爪蟾Key words
Peptidoglycan recognition protein/ Gene clone/ Expression analysis/ Xenopus tropicalis分类
生物科学引用本文复制引用
齐志涛,张启焕,王资生,王爱民,黄贝,昌鸣先,聂品..非洲爪蟾中一种长型肽聚糖识别蛋白的克隆与表达分析[J].动物学研究,2011,32(4):371-378,8.基金项目
This study was financially supported by the Project from the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (10KJB240001),the Foundation for Talent Recruitment of Yancheng Institute of Technology (XKR2011007),and the National Natural Science Foundation of China (30830083) （10KJB240001）
