中国实用儿科杂志2011，Vol.26Issue(9)：671-674,678,5.
肺泡表面活性物质B-18基因多态性与新生儿呼吸窘迫综合征易感性关系分析
Relationship between gentic polymorphism of surfactant protein B (SPB)-18 and neonatal respiratory distress syndrome susceptibility.
摘要
Abstract
Objective To study the relationship between gene polymorphism of Surfactant Protein B (SPB) -18 and neonatal respiratory distress symdrome susceptibility. Methods Totally 100 neonates diagnosed with NRDS and 186 gestational age, gender and birth weight matched neonates were enrolled from May. 2009 to Dec. 2010. The genetic polymorphism of SPB-18 was analyzed with high resolution melting (HRM) and gene sequence. Non-brochoscopic bronchoalveolar lavage( BALF )was performed from May 2009 to Dec. 2010 in 31 babies with SPB-18AC and 36 babies with SPB-18CC in Wuhan Children' s hospital. The levels of SPB in BALF were measured using Western blot(WB). Statistical analyses were carred out using SPSS software .version 13.0. Results The SPB-18AA, AC and CC genotype frequencies in NRDS patients were 11.0%, 40.0% and 49.0%,which were 6.5%, 31.7% and 61.8% in control group.P > 0.05, with no significant difference .The frequencies of A allele was higher in NRDS group than that in control group, (31.0% vs 22.3%) ,P < 0.05. There was difference in amounts of SPB in BALF from SPB-18 CC and AA individuals .The optical density was 14339.2 ± 1076.3 vs 8002.3 ± 452.9, P < 0.05. Conclusion Our study suggests that SPB-18 polymorphism may confer susceptibility to DRDS in Chinese. Differences in amounts of SPB resulting from the promoter SNP could affect the clinical presentation of NRDS.关键词
肺表面活性物质/基因多态性/新生儿呼吸窘迫综合征/易感性Key words
surfactant protein/ gene polymorphism/ neonatal respiratory distress syndrome/ susceptibility分类
医药卫生引用本文复制引用
曾凌空,李文斌,刘伟,刘汉楚,单瑞艳,常立文..肺泡表面活性物质B-18基因多态性与新生儿呼吸窘迫综合征易感性关系分析[J].中国实用儿科杂志,2011,26(9):671-674,678,5.基金项目
国家自然科学基金资助项目(30872795) （30872795）
