癌变·畸变·突变2011，Vol.23Issue(5)：344-348,5.DOI:10.3969/j.issn.1004-616x.2011.05.005
三氯乙烯致敏豚鼠肝脏损害研究
Liver damage induced by trichloroethylene in sensitized guinea pigs
摘要
Abstract
OBJECTIVE: To observe the changes of liver function and ultrastructures induced by trichloroethylene in sensitized guinea pigs. METHODS: While female guinea pigs (250-300 g) were randomly divided into blank control group, solvent (olive oil) control group and TCE treatment group. Guinea pigs were treated with guinea pig maximization test (GPMT). Specimens were collected at 24 h and 72 h after last stimulation, liver tissue pathology and ultrastructures examined, and AST, ALT, TP, ALB, GLB and A/G in serum measured. RESULTS: Sensitization rates were 65.38% in TGE treatment groups. Compared with TCE sensitized 24 h group and TCE non-sensitized 72 h group, ALT and AST levels in TCE sensitized 72 h group increased signiiicanlly(P<0.05). Compared with solvent group and TCE non-sensitized 72 h group, ALB levels in TCE sensitized 72 h group increased significantly(P<0.05). TCE sensitized group 72 h revealed more edema in liver cells with nucleus ruptured and disintegrated. TEM observation showed: a few mitochondria vacuoles, degeneration, rough endoplasmic reticulum decreased in TCE non-sensitized group. TCE sensitized group demonstrated reduction in the number of mitochondria in liver cells, rough endoplasmic reticulum fracture, expansion, decreased glycogen granules. In the group of 72 h after the last challenge, more serious damage was visible than those in 24 h group. CONCLUSION: Liver dysfunction and ultrastructural damage occurred in sensitized guinea pigs induced by trichloroelhylene. The extent of injury gradually increase with time.关键词
三氯乙烯/豚鼠/肝脏/损害Key words
trichioroethylene/ guinea pigs/ liver/ injury分类
医药卫生引用本文复制引用
朱启星,徐辉,冷静,沈形..三氯乙烯致敏豚鼠肝脏损害研究[J].癌变·畸变·突变,2011,23(5):344-348,5.基金项目
国家自然科学基金资助项目(30872147) （30872147）
教育部科学技术研究重点项目(210100) （210100）
安徽省自然科学基金项目(11040606M213) （11040606M213）
安徽高校省级自然科学研究重点项目(KJ2009A73) （KJ2009A73）
