癌变·畸变·突变2011，Vol.23Issue(5)：349-352,4.DOI:10.3969/j.issn.1004-616x.2011.05.006
三氯乙烯对肝细胞代谢酶基因与凋亡基因表达的影响
mRNA expression of hepatic metabolic enzyme genes and apoptosis genes in L02 cells treated with trichloroethylene
摘要
Abstract
OBJECTIVE: To study Mrna expression of hepatic metabolic enzyme genes and apoplosis genes in L02 cells after treatment with trichloroethylene (TCE). METHODS; liver cells (L02 cells) were cultured with various dosea of TCE (0.25, 0.5. 1.0 and 2.0 mmol/L) for 24 h, or with a single dose of TCE (1.0 mmol/L)for different periods of lime (3 h, 6 h, 12 h, 24 h), the conlrol was treated with DMSO. Real-time fluorescent PCR assay was applied for detection of Mrna expression of hepatic metabolic enzyme genes (CYP1A2, CYP3A4, CYP2E1) and apoptosis genes (BAX, BAD, Bcl-2). RESULTS: The relative levels of Mrna expression of hepatic metabolic enzyme genes and apoptosis genes markedly increased after TCE treatment at various doses for 24 h. The elevation of Mrna expression in TCE treatment groups was significant in comparison with control (P<0.05, or P<0.01}. Additionally, when the cells were treated with a single dose of TCE (1.0 mmol/L) for different periods of time, the Mrna expression also increased significantly compared with control (P<0.05, or P<0.0l). CONCLUSION; Trichloroelhylene could induce changes in Mrna expression of hepatic metabolic enzyme genes and apoptosis genes. These genes might play important roles in cytotoxicity of trichloroethylene.关键词
三氯乙烯/L-02肝细胞/代谢酶/凋亡基因Key words
trichloroethylene/ L02 cells/ metabolic enzyme/ apoptosis genes分类
生物科学引用本文复制引用
徐新云,洪文旭,刘月峰,柯跃斌,毛吉炎,袁建辉,周丽,杨淋清,杨细飞,黄海燕..三氯乙烯对肝细胞代谢酶基因与凋亡基因表达的影响[J].癌变·畸变·突变,2011,23(5):349-352,4.基金项目
深圳市科技计划项目(200802073) （200802073）
