中国医科大学学报2011，Vol.40Issue(8)：719-721,3.DOI:21-1227/R.20110726.1656.006
BMI-1基因和蛋白在子宫颈癌中的表达和临床意义
Expression and Clinical Significance of BMI-1 Oncogene and Its Protein in Cervical Cancer
摘要
Abstract
Objective To investigate the expression of B cell-specific MLV insertion site-1 (BMI-1) and its clinicopathologic significance in cervical cancer. Methods The expression of BMI-1 mRNA was detected by real time polymerase chain reaction (PCR) in 30 cervical cancer samples and 10 normal cervical samples. The expression of BMI-1 protein was examined by SP immunohistochemistry in archival paraffin-embedded tissue specimens. Statistical analysis was performed to investigate the association between the clinicopathologic features and BMI-1 expression. Results Real time-PCR demonstrated that the BMI-1 mRNA levels in cervical cancer samples were significantly higher than those in normal cervical samples (P< 0.05). Immunohistochemical staining showed that BMI-1 protein was highly expressed in cervical cancer tissues than normal cervical tissue (P < 0.05). Expression of BMI-1 protein was highly correlated with the differentiation degree of tumor and lymph node metastasis (P < 0.05). No significant difference of BMI-1 protein expression was found between cervical squamous cell carcinoma and adenocarcinoma (P > 0.05). Conclusion Overexpression of BMI-1 plays an important role in the oncogenesis and development of cervical cancer. Expression of BMI-1 protein was highly correlated with the differentiation degree of tumor and lymph node metastasis.关键词
子宫颈癌/B细胞特异的莫洛尼白血病毒插入位点1基因/免疫组化/实时定量PCRKey words
cervical cancer/ B-cell specific moloney murine leukemiavirus insertion site 1/ immunohistochemistry/ real time-PCR分类
医药卫生引用本文复制引用
高翔,张淑兰..BMI-1基因和蛋白在子宫颈癌中的表达和临床意义[J].中国医科大学学报,2011,40(8):719-721,3.基金项目
国家自然科学基金资助项目(30973191),辽宁省科学技术计划(2008225004) （30973191）
盛京自由研究者计划(200806) （200806）
