安徽农业科学2011，Vol.39Issue(16)：9503-9504,9521,3.
适于AFLP分析用的桃韧皮部DNA提取方法
DNA Extraction Method of Peach Phloem Suitable for AFLP Analysis
王富荣 1何华平 2赵剑波 1龚林忠 2王会良3
作者信息
- 1. 湖北省农业科学院果树茶叶研究所,湖北,武汉,430209
- 2. 湖北省农业科技创新中心果树茶叶分中心,湖北,武汉,430209
- 3. 北京市农林科学院林业果树研究所,北京,100093
- 折叠
摘要
Abstract
[Objective] The research aimed to study the peach phloem DNA extraction method which was suitable for AFLP analysis,which laid the foundation for the peach genome research when the spire and other young materials couldn't be obtained in winter. [ Method] The phloem of eight kinds of wild peach dormant wood in one year old in winter was the test material. The genome total DNA was extracted by u-sing the improved CTAB method,and the extraction effect was compared with that in the corresponding spire DNA. Moreover,AFLP verified a-nalysis was carried out. [Result] Except PNA yield which was extracted from the wild peach phloem was lower than that in the spire,DNA purity and integrity were good. OD260/OD280 was during 1.8-2. 0,and there was no degradation phenomenon. RNA removal was clean,and it could be completely digested by the restriction endonuclease. AFLP analysis band was clear,and the polymorphism was good. [ Conclusion] The wild peach phloem genome total DNA which was extracted by the method was entirely suitable for AFLP analysis.关键词
桃/韧皮部/DNA提取/AFLP分析Key words
Peach/ Phloem/ DNA extraction/ AFLP analysis分类
农业科技引用本文复制引用
王富荣,何华平,赵剑波,龚林忠,王会良..适于AFLP分析用的桃韧皮部DNA提取方法[J].安徽农业科学,2011,39(16):9503-9504,9521,3.
