中国药房2011，Vol.22Issue(29)：2711-2713,3.
氯沙坦对进展性肾炎模型大鼠肾小管-间质细胞低氧诱导因子及结缔组织生长因子的影响研究
Effects of Losartan on Tubulointerstitial Hypoxia Inducible Factors and Connective Tissue Growth Factors in Progressive Nephritis Model Rats
摘要
Abstract
OBJECTIVE: To probe into the effects and mechanism of losartan on tubulointerstitial hypoxia inducible factor-1α (HIF-1α) and connective tissue growth factor (CTGF)in progressive nephritis model rats. METHODS: The rats underwent right ne-phrectomy and was injected with IgG(OX-7) mouse monoclonal anti-Thyl.l antibody twice to establish progressive nephritis model. Then some model rats were included in model group and other rats were divided into control group and losartan high-dose and low-dose groups (80, 20 mg·kg-1·d-1), some rats were selected and included in sham operation group with 5 rats in each group. Above three groups were given relevant drugs for four weeks intragastrically. The levels of 24 h urine protein, serum creatinine, HIF-1α Mrna and CTGF Mrna and pathological changes of kidney were observed. RESULTS: Compared with sham operation group, serum creatinine, 24 h urine protein, renal interstitium area, HIF-1α Mrna and CTGF Mrna expression in model groups increased significantly (P<0.01 or P<0.001); compared with model group, serum creatinine and CTGF Mrna expression in losartan high-dose and low-dose groups decreased significantly (P<0.05 or P<0.01), while 24 h urine protein, renal interstitium area and HIF-la Mrna level in losartan high-dose group decreased significantly (P<0.01) and had no obvious change in losartan low-dose group. Pathological observation of model group showed that inflammatory cell infiltration and renal fibrosis. Tubulointerstitial injury was ameliorated in losartan high-dose and low-dose groups, compared with control group. CONCLUSIONS: Losartan may protect kidney by decreasing the expression of HIF-1α Mrna and CTGF Mrna and tubulointerstitial fibrosis in the progressive nephritis model rats.关键词
氯沙坦/进展性肾炎/大鼠/低氧诱导因子-1α/结缔组织生长因子Key words
Losartan/Progressive nephritis/Rats/Hypoxia inducible factor-la/Connective tissue growth factor分类
医药卫生引用本文复制引用
查艳,何平红,龙艳君,袁静,俞雷..氯沙坦对进展性肾炎模型大鼠肾小管-间质细胞低氧诱导因子及结缔组织生长因子的影响研究[J].中国药房,2011,22(29):2711-2713,3.基金项目
贵州省优秀科技教育人才省长专项资金项目(黔省专合字(2010)89号) （黔省专合字(2010）
贵州省“十一五”社会发展科技项目(黔科合SY字[2008]3058) （黔科合SY字[2008]3058）
