摘要
Abstract
OBJECTIVE: To investigate the effect of Curcumin-loaded solid lipid nanoparticles (Cur-SLN) alone or combined with DDP on the growth of human ovarian cancer SKOV3 cells. METHODS: Single drug test groups were divided into blank control group, Cur group and Cur-SLN group (the concentration of Cur were 10, 20, 40, 60, 80 umol-L"1). The drug combination test groups were divided into blank control group, DDP group, DDP+Cur group and DDP+Cur-SLN group (the concentration of DDP were 1, 2, 3, 4, 5 nmol-L"1, that of Cur was 10 umol-L~'). The inhibition rate of cell growth in above groups after 24 h, 48 h and 72 h were determined. Another group was divided into blank control group, DDP group, Cur group, Cur-SLN group, DDP+Cur group and DDP+Cur-SLN group(the concentration of DDP was 2.5 umol-L1, that of Cur was 10 umol-L1). The apop-tosis rate of SKOV3 cell after 24 h and the expression of cell cycle regulation factor Cyclin E, CDK2 of above groups were determined. RESULTS: With same concentration, the inhibition rate of SKOV3 cell growth in Cur-SLN group after different treatment time increased significantly, compared with Cur group (P<0.05). Compared with DDP group, the inhibition rate of SKOV3 cell growth in DDP+Cur group and DDP+Cur-SLN group increased significantly(P<0.05), that of DDP+Cur-SLN group was significantly higher than DDP+Cur group (P<0.05). Compared with blank control group, the other 5 groups could more significantly cause G2 phase arrest; the apoptosis rate and the expression of Cyclin E, CDKz were increased significantly (P< 0.05 or f<0.01), and those of DDP+Cur-SLN group were more significantly higher than other groups(P<0.05). CONCLUSIONS: Cur-SLN can significantly inhibit the proliferation of human ovarian cancer SKOV3 cells and induce cell apoptosis, and shows synergistic effects when combined with DDP.关键词
姜黄素/固体脂质纳米粒/卵巢癌SKOV3细胞/顺铂：单用/联用Key words
Curcumin/Solid lipid nanoparticles/Ovarian cancer cell line SKOV3/DDP/ Alone/Combine分类
医药卫生
