中国药房2011，Vol.22Issue(29)：2748-2750,3.
HPLC法同时测定复合维生素口服液中4种水溶性维生素的含量
Simultaneous Determination of 4 Kinds of Water-soluble Vitamins in Compound Vitamin Oral Solution by HPLC
白靖 1向柏 1申磊 1何朝星 1张贵琴 1曹德英1
作者信息
- 1. 河北医科大学药学院,石家庄市050017
- 折叠
摘要
Abstract
OBJECTIVE: To establish a method for simultaneous determination of 4 kinds of water-soluble vitamins in Compound vitamin oral solution. METHODS: HPLC method was adopted. The content of 4 kinds of water-soluble vitamins, including nicotinamide, vitamin B6, vitamin B1 and vitamin B2 sodium phosphate, were determined by HPLC. A Diamonsil C18 column was used. The mobile phase consisted of sodium hexanesulfonate solution-methanol-acetic acid (690:303: 7, VIVIV) and the UV detection wavelength was 280 nm. RESULTS: 4 components were separated well and the calibration curves showed good linearity within the range of 87.5 -700.0μg·mL-1 for nicotinamide, 7.5 - 60.0 μg·mL-1 for vitamin Be, 25 - 200 (μg·mL-1 for vitamin B1, and 17.5 - 140.0 μg·rnL-1 for vitamin B2 sodium phosphate, respectively (r>0.999 3). The average recoveries were 101.3% (RSD= 0.5% ), 99.5% (RSD=0.6% ), 100.0% (RSD=0.6% ), 98.4% (RSD=0.9% ), respectively. CONCLUSION: The proposed method is convenient, fast, accurate and reproducible. It provides reference for quality control of vitamins components of compound vitamin formulations and health product.关键词
高效液相色谱法/烟酰胺/维生素B6/维生素B1/维生素B2磷酸酯钠/含量测定Key words
HPLC/Nicotinamide/Vitamin B6/Vitamin B1/Vitamin B2 sodium phosphate/Content determination分类
医药卫生引用本文复制引用
白靖,向柏,申磊,何朝星,张贵琴,曹德英..HPLC法同时测定复合维生素口服液中4种水溶性维生素的含量[J].中国药房,2011,22(29):2748-2750,3.
