安徽农业科学2011，Vol.39Issue(16)：9600-9601,2.
HPLC谱法测定蒲公英不同器官中绿原酸的含量
Determination of Chlorogenic Acid of Different Organs from Taraxacum mongolicum by High Performance Liquid Chromatography
陈萍 1王建刚2
作者信息
- 1. 吉林化工学院化学与制约工程学院,吉林,吉林,132022
- 2. 吉林化工学院分析测试中心,吉林,吉林,132022
- 折叠
摘要
Abstract
[ Objective ] To establish a method for determining contents of chlorogenic acid in different organs from Taraxacum mongolicum by HPLC. [Method] The HPLC was carried out with Shim-pack VP-ODS column (4.6 mm × 150.0 mm,5 μm) using mixture of methanol and 0.4 % phosphoric acid solution in different ratio as mobile phase. Other conditions were: flow rate of 1.0 ml/min, detection wavelength of 326 nm, column temperature of 40 ℃ and sampling amount of 20 μl. [ Result ] When the concentration of chlorogenic acid ranged from 0.791 2 to 79.120 0 μg/ml, the linear relationship between concentration and peak area was good (r =0.999 8). The recovery was 98.00% -101.50%. Leaves、 roots、 flowers and scapes of Taraxacum mongolicum were 1.212, 0.536, 0.448 and 0.417 mg/g in chlorogenic acid, respectively. [ Conclusion] The method established in this work was a rapid and accurate method for determining chlorogenic acid from Taraxacum mongolicum. Leaf of Taraxacum mongolicum was highest in chlorogenic acid, which provided basis for better developing and applying of Taraxacum mongolicum.关键词
蒲公英/不同器官/绿原酸/HPLCKey words
Taraxacum mongolicum/ Different organs/ Chlorogenic acid/ HPLC分类
农业科技引用本文复制引用
陈萍,王建刚..HPLC谱法测定蒲公英不同器官中绿原酸的含量[J].安徽农业科学,2011,39(16):9600-9601,2.
