中国普通外科杂志2011，Vol.20Issue(7)：713-716,4.
β-catenin在L-02,WB,HepG2和SMCC-7721细胞中的表达及其意义
Expression and significance of β-catenin in the L-02, WB, HepG2 and SMCC-7721 cells
申海军 1陈广瑜 1詹建兴 1胡波 1金世龙1
作者信息
- 1. 西安航天总医院肝胆外科,陕西 西安710100
- 折叠
摘要
Abstract
Objective To investigate the abnormal expression of B-catenin and its role in the development and differentiation of primary hepatocellular carcinoma. Methods Four types of cell lines ( L-02 , WB , HepG2 and SMCC-7721 ) were attachment cultured and subcultured. The expression characteristics of B-catenin in those cells were detected by immunocytochemical staining. Results Of the L-02 cells, the B-catenin proteins were expressed totally on the cell membrane and none of them were expressed in the cytoplasm and nucleus. Of the WB cells, the B-catenin proteins were expressed mainly on the cell membrane and slightly expressed in the cytoplasm and nucleus. However, the B-catenin proteins were strongly and totally expressed in the cytoplasm and nucleus of HepG2 and SMCC-7721 cells, and the B-catenin expressions in SMCC-7721 cells were higher than those of the HepG2 cells. Conclusions The translocated expression of B-catenin from the cell membrane to cytoplasm and nucleus may play an important role in the development and differentiation of primary hepatocellular carcinoma.关键词
癌,肝细胞/β-catenin/人肝细胞株/人肝干细胞株/人肝癌细胞株/SMCC-7721细胞株/免疫细胞化学Key words
Carcinoma, Hepatocellular/ B-catenin/ L-02 Cells/ WB Cells/ HepG2 Cells/ SMCC-7721 Cells/ Immunocytochemistry分类
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申海军,陈广瑜,詹建兴,胡波,金世龙..β-catenin在L-02,WB,HepG2和SMCC-7721细胞中的表达及其意义[J].中国普通外科杂志,2011,20(7):713-716,4.
