中国普通外科杂志2011，Vol.20Issue(7)：725-730,6.
EGCG抑制IL-6诱导肿瘤血管生成的作用及机制
Epigallocatechin-3-gallate inhibits angiogenesis of gastric cancer induced by IL-6 and its mechanism
摘要
Abstract
Objective To investigate the inhibitory effect of ( - ) - Epigallocatechin-3-gallate ( EGCG) on angiogenesis of gastric cancer induced by IL-6 and its molecular mechanism. Methods ACS gastric cancer cells were cultured with IL-6 (50 ng/mL) or with a series of concentrations of EGCC. Protein levels of vascular endothelial growth factor ( VEGF) in tumor cells were examined by Western blot, the VEGF release in tumor culture medium was determined by ELISA , and VEGF mRNA expression in tumor cells by RT-PCR. The conditioned media from gastric cancer cells were prepared for culturing human umbilical vein endothelial cells ( HUVEC ) . Vascular endothelial cell proliferation was measured with MTT assay, in vitro angiogenesis was determined with endothelial cell tube formation assay in Matrigel, and angiogenesis in vivo was measured with Matrigel plug assay. Results The VEGF protein level, release and mRNA expression were increased in the IL-6 induced AGS cells by 2. 4, 2. 8 and 3. 1 folds, respectively. EGCG treatment significantly inhibited the IL-6 induced increase in level and release of VEGF protein and in mRNA expression in a dose-dependent manner. The proliferation and tube formation of vascular endothelial cells induced by IL-6 in vitro were markedly inhibited by VEGF neutralizing antibody and EGCG. The angiogenesis in vivo induced by IL-6 was also markedly inhibited by VEGF neutralizing antibody and EGCG. Conclusions EGCG reduces expression of VEGF induced by IL-6 in gastric cancer cells, and thereby inhibits tumor angiogenesis.关键词
胃肿瘤/表没食子儿茶素没食子酸酯/血管生成/血管内皮生长因子Key words
Stomach Neoplasms/ Epigallocatechin-3-gallate/ Angiogenesis/ Vascular Endothelial Growth Factor分类
医药卫生引用本文复制引用
朱宝和,何裕隆,詹文华,王昭,张常华,詹勇强..EGCG抑制IL-6诱导肿瘤血管生成的作用及机制[J].中国普通外科杂志,2011,20(7):725-730,6.基金项目
中国博士后基金资助项目(20100470963). （20100470963）
