实用医学杂志2011，Vol.27Issue(19)：3485-3487,3.DOI:10.3969/j.issn.1006-5725.2011.19.013
环介导等温扩增技术检测粪便幽门螺杆菌方法的建立及应用
Development and clinical application of loop-mediated isothermal amplification method on feces Helicobacter pylori detection
摘要
Abstract
Objective To develop a loop-mediated isothermal amplification (LAMP) method for detecting Helicobacter pylori (Hp) of feces rapidly and simply. Methods Four specific LAMP primers (two inner primers and two outer primers) were designed by targeting 16S rRNA gene of Hp. After optimizing reaction condition, the sensitivity and specificity of the method were evaluated by simulated feces samples detection. Results The method could be finished within 2 h, and positive results were green and had a ladder-like pattern on the gel. The sensitivity of Hp genome DNA was 12 pg, and was 10 times as much as PCR, the detection limit of feces simulated samples was 102 CFU/mL. The results of Staphylococcus aureus ^ Escherichia coh\ Pseudomonas aeruginosa and Enterococcus facealis were negative. Conclusion LAMP assay is high sensitive, specific and simple to operate with no need special equipments, which can be taken as a promising method for Hp detection.关键词
螺杆菌,幽门/环介导等温扩增技术/16s rRNAKey words
Helicobacter pylori/ Loop-mediated isothermal amplification/ 16S rRNA引用本文复制引用
韩慧,胡子有,姚芳,曾勇,吴炳义,熊婧..环介导等温扩增技术检测粪便幽门螺杆菌方法的建立及应用[J].实用医学杂志,2011,27(19):3485-3487,3.基金项目
广东省"211工程"三期重点学科建设项目 （）
