安徽农业科学2011，Vol.39Issue(16)：9985-9987,3.
绵羊卵母细胞蛋白质组2-DE图谱的构建及初步分析
Construction and Preliminary Analysis of Ovine Oocytes proteomic 2-DE Map
摘要
Abstract
[Objective] To obtain ovine oocytes 2-dimensional gel electrophoresis (2-DE) maps. [Method] Ovine oocytes were solubilized in lysis buffer,and protein concentrations were measured using the method of Bradford and Ramagli. Then the samples were subjected to two-dimensional polyacrylamide gel electrophoresis. The 2-DE maps of ovine oocytes were analyzed through PDQuest8. 0. [ Result ] The Ramagli method could reflect concentration of oocyte protein more actually and could optimize the quantity of samples to get a 2-DE map with higher quality. The protein concentration measured by the Bradford method was higher than its actual value. [ Conclusion] Each 2-DE map loading 700 oocytes is more reasonable.关键词
绵羊/卵母细胞/双向电泳/蛋白质组Key words
Ovine/ Oocytes/ Two-dimensional polyacrylamide gel electrophoresis/ Proteome分类
生物科学引用本文复制引用
林雍峰,张勇,李鸿岩,胡俊杰,赵兴绪..绵羊卵母细胞蛋白质组2-DE图谱的构建及初步分析[J].安徽农业科学,2011,39(16):9985-9987,3.基金项目
科技部国际合作重点项目计划(2005DFA30720). （2005DFA30720）
