临床口腔医学杂志2011，Vol.27Issue(9)：523-526,4.DOI:10.3969/j.issn.1003-1634.2011.09.004
高强度周期性牵张应力促进体外培养成肌细胞凋亡的机制探讨
The mechanism of myoblasts apoptosis under high stretch
摘要
Abstract
Objective: To investigate the apoptosis mechanism of myoblasts C2C12 under cyclic stretch. Method: Flexercell Strain Unit was introduced to load cyclic stretch. DNA ladder experiment was used to test the apoptosis when they were loaded different stretches. Western blot was introduced to test pJNK1 level and NFkappaB reporter showed their activity. JNK1 RNAi was introduced to rescue NFkappaB. Result: When C2C12 cells were under 10 % cyclic stretch they began Spindle slightly longer. DNA ladder experiment showed when the stretch was more than 15 % it began to apoptosis. During this process pJNK1 increased while NFkappaB was inhibited. JNK1 RNAi could rescued NFkappaB inhibition. Conclusion: pJNK1 was increased when C2C12 myoblasts apoptosis under high stretch while NFkappaB was inhibited.关键词
周期性牵张应力/成肌细胞/凋亡/JNK1/NFKappa BKey words
cyclic stretch/myoblast/apoptosis/JNK1/NFKappaB分类
医药卫生引用本文复制引用
谭家莉,匡威,段建民,毕媛,汪维建,李潇,段银钟,刘彦普..高强度周期性牵张应力促进体外培养成肌细胞凋亡的机制探讨[J].临床口腔医学杂志,2011,27(9):523-526,4.基金项目
国家自然科学基金资助(30900860)国家公派留学专项研究生奖基金项目(2009659007) （30900860）
