临床小儿外科杂志2011，Vol.10Issue(5)：329-332,336,5.DOI:10.3969/j.issn.1671-6353.2011.05.004
血清蛋白质标记物在神经母细胞瘤中的诊断意义
Clinical application of serum protein markers in neuroblastoma
摘要
Abstract
Objective To search the specific protein biomarkers constructing the serum protein markers in the early dignosis of Neuroblastoma. Methods Eighty-seven serum samples (including 47 Neuroblastoma patients, 30 children malignant solid tumor 10 healthy children)were detected by SELDI-TOF-MS, and proceeded by ZUCI-protein chip Data Analyze System Software to be identified by Jackknife, and evaluate the model' s predictive effect. Results To analyze all the serum protein icon by cluster analysis can find a proteomics model. The detective model in combined with 10 biomarkers could separate neuroblastoma from the healthy group with sensitivity of 100. 00%, specificity of 100.00%; separate preoperative neuroblastoma from postoperative neuroblastoma with sensitivity of 100.00%, specificity of 100.00% ;The diagnosis model combined with 5 biomarkers could separate neuroblastoma from other children malignant solid tumors with sensitivity of 88. 89%, specificity of 100. 00%. Conclusion This detective model is a more effective method for the early diagnosis neuroblastoma, and can develop to a new path to research for new tumor markers to study the proteomics in neuroblastoma. The model of neuroblastoma combinated by SELDI-TOF-MS with SVM and bioinformation technique can regard as another more specificity and sensitivity auxiliary examination mean.关键词
血清蛋白类/神经母细胞瘤/诊断Key words
Blood Proteins/Neuroblastoma/DI引用本文复制引用
陈新让,王家祥..血清蛋白质标记物在神经母细胞瘤中的诊断意义[J].临床小儿外科杂志,2011,10(5):329-332,336,5.基金项目
本研究为国家自然科学基金资助项目(项目号30571930) （项目号30571930）
