生物信息学2011，Vol.9Issue(3)：205-209,5.DOI:10.3969/j.issn.1672-5565.2011.03.007
垂体GnRHR,FHS-β和LH-β基因表达与GnRHR生物信息学研究
Researches of Gene Expression of Pituitary GnRHR,FSH-β and LH-β in Female Rabbits and GnRHR Bioinformatics
摘要
Abstract
The objectives of the study is to explore gene expression of pituitary GnRHR, FSH - p and LH - p after injecting GnRH -A in female rabbits. Twenty four rabbits (Oryctolagus cuniculus) were divided into EG - I , EG -II, EG - III and control group (each of 6 rabbits) . Rabbits in EG - I , EG -II and EG-III were subcutane-ously injected with 1.0 mL( 100?g/mL,100?g/mL and 50?g/mL) GnRH -A antigen respectively. EG - II and EG - III was re - injected on d 20 at the origin dose. Control group was as blank. The results showed that GnRHR, FSH -β and LH - β exist in the pituitary of female rabbits. GnRHR, FSH -β and LH-β AACt in EG -II and EG-III were higher than that in EG - I (P < 0.05). The homologies of GnRHR, FSH-β and LH -β sequences were respectively 98% , 100% and 94% with those reported in NCBI. In conclusion, GnRH - A at the different dosages may influence obviously the gene expression of GnRHR, FSH-β and LH-β mRNA in rabbit s pituitary, re - injection of alarelin had more effects.关键词
促性腺激素释放激素激动剂/GnRH受体/垂体/基因表达/生物信息学/雌兔Key words
Gonadotropin releasing hormone agonist/GnRH receptor/pituitary/gene expression/bioinformatics/female rabbit分类
农业科技引用本文复制引用
魏锁成,巩转娣,韦敏..垂体GnRHR,FHS-β和LH-β基因表达与GnRHR生物信息学研究[J].生物信息学,2011,9(3):205-209,5.基金项目
国家自然科学基金项目(编号:31060350) （编号:31060350）
国家民委2007年度重点资助项目(No.MW2007-ZD-027). （No.MW2007-ZD-027）
