实用医学杂志2011，Vol.27Issue(19)：3495-3497,3.DOI:10.3969/j.issn.1006-5725.2011.19.017
靶向phTERT-PE38KDEL重组质粒联合硼替佐米抑制裸鼠胰腺癌移植瘤生长的研究
Effects of recombinant plasmid phTERT-PE38KDEL combined bortezomib on inhibiting the transplanted pancreatic tumor growth in athymic mice
邢帅 1孙晋津 1李宝玉 1孙宁 1陈剑秋1
作者信息
摘要
Abstract
Objective To explore the effects of recombinant plasmid phTERT-PE38KDEL and bortezomib on the growth of transplanted pancreatic tumor in athymic mice. Methods Human pancreatic carcinoma cell line MiaPaCa2 was planted into nude mice to establish the xenografts tumor model By intraperitoneal administration and intratumoral injection, the xenografts tumor mice were treated with bortezomib combined phTERT-PE38KDEL. The tumor volume and weight in all groups were measured, and the tumor inhibitory rate was calculated. The proliferating cell nuclear antigen and microvascular density were examined by immunohistochemistry. Cell apoptosis was observed by TUNEL assay. Results The tumor volume and weight of the combined therapeutic group (Group A) was significantly smaller than those of the gene therapeutic group (Group B), chemotherapy group (Group C) and control group (Group D) at each time point (P < 0.01). Compared with Group D, the inhibitory rate of tumor growth in Group A, B and C were 68.98%, 51.44% and 16.39%, respectively. The PCNA proliferation index and MVD of Group A were significantly lower than those of Group B and C (P < 0.01). Compared with Group B and C, the apoptosis cells in Group A were significantly increased(P < 0.01). Conclusion Both phTERT-PE38KDEL and bortezomib have antitumor effects in vivo and the combined therapy has a stronger antitumor effect.关键词
胰腺肿瘤/hTERT启动子/PE38KDEL基因/硼替佐米Key words
Pancreatic neoplasms/ hTERT promoter/ PE38KDEL gene/ bortezomib引用本文复制引用
邢帅,孙晋津,李宝玉,孙宁,陈剑秋..靶向phTERT-PE38KDEL重组质粒联合硼替佐米抑制裸鼠胰腺癌移植瘤生长的研究[J].实用医学杂志,2011,27(19):3495-3497,3.
