中国骨伤2011，Vol.24Issue(10)：838-840,3.DOI:10.3969/j.issn.1003-0034.2011.10.011
新西兰大白兔骨膜下组织工程骨异位成骨的实验研究
Experimental study of ectopic bone formation of engineered bone constructs under the periosteum of New-Zealand rabbits
周强 1汪洋 2虞杰 3余迎浩2
作者信息
- 1. 上海中医药大学附属普陀医院骨科,上海,200062
- 2. 舟山市中医骨伤联合医院骨科
- 3. 复旦大学上海医学院解剖与组织胚胎学系
- 折叠
摘要
Abstract
Objective: To study the feasibility of ectopic bone formation for engineered bone constructs with bone marrow stromal cells (BMSCs). Methods:BMSCs were obtained from 3-month female New-Zealand rabbits with weight of 3 kg,induced to osteogenitor cell, were expanded by culture and then seeded into the porous p-tricalcium phosphate ((3-TCP) particles. Engineered bone constructs were implanted under the periostem of rabbit femur. Samples were retrieved and examed after 3 months. Blood vessels and osteoblast were examined through optical microscope. Results: Twelve implanted engineered bone particles of 16 samples were fixed well under periosteum and rounded by periosteum. There were a lot of vessels and new bone in engineered bone. The structure of bone was disorder;the vessels arranged equally. Four cases found implanted bone freed outside of periosteum, lots of implanted material were absorbed, the volume of residual was less than osteogenesis, and lack of blood vessel. 80% engineered bone constructs attached to the femur under the periostem very well, osteogenesis was fine and vessels were growed into new bone. Conclusion: Engineered bone can obtained good ectopic bone under the periostem.关键词
组织工程/骨膜/干细胞/动物,实验Key words
Tissue engineering/ Periosteum/ Stem cells/ Animals,laboratory引用本文复制引用
周强,汪洋,虞杰,余迎浩..新西兰大白兔骨膜下组织工程骨异位成骨的实验研究[J].中国骨伤,2011,24(10):838-840,3.
