中国比较医学杂志2011，Vol.21Issue(9)：54-57,封4,5.DOI:10.3969/j.issn.1671.7856.2011.09.013
猪细小病毒单克隆抗体的制备和鉴定
Preparation and Characterization of Monoclonal Antibody against Porcine Parvovirus
摘要
Abstract
Objective Preparation and characterizing of porcine parvovirus (PPV) bybridomas cells and their monoclonal antibody. Methods Two hybridomas cells were obtained by conventional methods.. The hybridomas cells were analysed and identified by chromosome analysis, indirect ELISA, the immunoperoxidase monolayer assay (IPMA) and indirect immunofluorescence (IFA) methods. Results Two hybridomas cells were obtained and named 2H9 and 1F9 respectively. The chromosome numbers were between 90 to 110. Titration of the hybridomas culture supernatant were 1:1 x 104, and titration of ascitic fluid were 1: 1 x 107, their subclasses were IgCl, IgM, belonging to kappa chain, and without cross-reaction with PRRSV,CSFV,PRV, PCV-1, PCV2 and JEV. Furthermore, the specific reaction of antibodies and PPV in PK-15 cells was confirmed IPMA and IFA. Conclusion Two anti-PPV hybridomas cells were acquired and the characterizations of two monoclonal antibodies were confirmed .关键词
猪细小病毒/单克隆抗体/免疫过氧化物酶单层试验/间接免疫荧光Key words
Porcine parvovirus/ Monoclonal antibodies/ IPMA/ IFA分类
农业科技引用本文复制引用
张倩,遇秀玲,翟新验,田克恭,李晓霞,董昕欣,顾小雪,曹振,邓小雨,胡冬梅,刘奇,周智..猪细小病毒单克隆抗体的制备和鉴定[J].中国比较医学杂志,2011,21(9):54-57,封4,5.基金项目
"十一五"国家科技支撑计划动物源性生物材料病毒检测技术研究(2008BAI54B06). （2008BAI54B06）
