工业微生物2011，Vol.41Issue(5)：1-5,5.DOI:10.3969/j.issn.1001-6678.2011.05.001
重组大肠杆菌表达氧化还原酶不对称还原2-羟基苯乙酮的研究
Asymmetric reduction of 2-hydroxyacetophenone by recombinant oxidoreductases expressed in Escherichia coli
摘要
Abstract
13 recombinant Escherichia coli BL21(DE3) overexpressing 13 different oxidoreductases were constructed respectively. 2-hydroxyacetophenone was applied as substrate to investigate the biocatalytic asymmetric reduction by the recombinants. The results indicated that relative enzyme activity of the expressed enzyme was much higher at the induction temperature of 17℃ than that at 30℃ and 37℃. Besides, SCR1, which was from Candida parapsilosis CCTC-CM203011 showed best performance. The effects of substrate concentration on asymmetric conversion of 2-hydroxyacetophenone were investigated. It could asymmetrically reduce α-hydroxyacetophenone to produce (S)-PED with 99.9% ee and 90.3% yield with substrate concentration of 5 g/L. By introducing ethyl laurate as organic phase to form biphasic system, 8 g/L 2-hydroxyacetophenone was reduce to (S)-PED with 99.9% ee and 86.9% yield.关键词
不对称催化还原/氧化还原酶/2一羟基苯乙酮/SCR1Key words
asymmetric reduction/ oxidoreductase/ a-hydroxyacetophenone/ SCR1引用本文复制引用
闫真,聂尧,徐岩..重组大肠杆菌表达氧化还原酶不对称还原2-羟基苯乙酮的研究[J].工业微生物,2011,41(5):1-5,5.基金项目
国家自然科学基金(20776060,30800017),863国家高技术研究发展计划(2006AA020104,2007AA02Z226),973国家重点基础研究发展规划(2009CB724706),高等学校学科创新引智计划(111计划,111-2-06),江苏省自然科学基金(BK2008528). （20776060,30800017）
