广东牙病防治2011，Vol.19Issue(9)：451-454,4.
模拟微重力培养环境下牙周膜干细胞生长状态的研究
Biological Behavior of Human Periodontal Ligament Stem Cells in Simulated Microgravity Environment MA
马兆峰 1李石 2牛忠英3
作者信息
- 1. 中国医科大学北京顺义医院口腔科,北京,101300
- 2. 中国人民解放军66055部队医院口腔科
- 3. 中国人民解放军第306医院口腔治疗中心
- 折叠
摘要
Abstract
Objective To investigate the growth status of human periodontal ligament stem cells (hPDLSCs) in simulated microgravity in vitro. Methods HPDLSCs were isolated and cultivated, then characterized by immunohistochemis-try of stromal cell antigen-1 ( STRO-1). After 21 days of induction, the results were evaluated by Alizarin red staining and oil' 0' staining. HPDLSCs were co-incubated with microcarrier beads of Cytodex-3, and were placed in rotary cell culture system. Cells morphology and proliferation potential were examined. Results HPDLSCs were cultivated, and growth characteristics and multipotent differentiation were assessed. The results showed that hPDLSCs can be cultured in simulated microgravity environment. On the 1st day (t =5. 590, P =0. 005), the 3rd day ( t = 12. 238, P =0.000) , the 5th day (t = 19.124, P = 0.000), the 7th day (t=35. 103, P =0.000), simulated microgravity statistically promoted the proliferation potential compared with cells in normal gravity environment. Conclusion The simulated microgravity culture system has the potential to be used for the bioengineering reconstruction of the periodontal tissues.关键词
牙周膜干细胞/多向分化潜能/模拟微重力Key words
Human periodontal ligament stem cells/ Multi-directional differentiation/ Simulated microgravity分类
生物工程引用本文复制引用
马兆峰,李石,牛忠英..模拟微重力培养环境下牙周膜干细胞生长状态的研究[J].广东牙病防治,2011,19(9):451-454,4.
