环境与职业医学2011，Vol.28Issue(9)：547-550,4.
氯化镨对小鼠骨髓细胞微核率和核异常率的影响及硒的干预作用
Effects of Praseodymium Chloride on Rates of Bone Marrow Micronuclei and Nucleus Abnormity Rate in Mice and Selenium Intervention
屈艾 1孙玲 1闫晶晶 2刘小燕 1沈益宏1
作者信息
- 1. 徐州师范大学生命科学学院环境毒理研究室,江苏,徐州,221116
- 2. 徐州工程学院,江苏,徐州,221008
- 折叠
摘要
Abstract
[Objective] To investigate the effects of Praseodymium Chloride (PC) on rates of bone marrow micronuclei and nucleus abnormity in mice, as well as possible interventional effect of sodium selenite. [ Methods ] Five to six-week old Kunming mice of were divided into 12 groups: five exposure groups with doses at 7.5, 15, 30, 60, 120mg/(kg·bw) of PC, five treatment groups with the same five PC doses added 0.05 mg/(kg·bw) Selenium respectively, negative(0.85% normal saline) and positive(sodium nitride, 20 mg/(kg·bw) groups. All doses were exposed via intraperitoneal injection and standard micronucleus test and nuclear test were followed. Mouse marrow cell frequency micronucleus(FMN)and frequency abnormal nuclei(FAN)were counted and the impacts of FMN and FAN after being added selenite sodium were observed. [ Results ] The 7.5-120 mg/(kg·bw) doses of PC induced a remarkable increase of FMN and FAN and showed a significant correlation between the dosages and their effects (rfmn=0.885, rFAN=0.914). In the pairwise comparison of the treatment groups and the exposure groups, the FMN and FAN decreased after adding sodium selenite (t-test, P<0.05 except in the 7.5mg/(kg·bw) PC groups). [ Conclusion ] FMN and FAN of mouse marrow cell nucleus increase by exposed to PC, which indicates PC with possible genetic damage effects. Furthermore, sodium selenite could have intervention effects to PC exposure by reducing FMN and FAN.关键词
氯化镨/亚硒酸钠/小鼠骨髓细胞/微核率/核异常率Key words
praseodymium chloride/ sodium selenite/ mouse bone marrow cells/ micronucleus/ abnormally nuclei frequency分类
化学化工引用本文复制引用
屈艾,孙玲,闫晶晶,刘小燕,沈益宏..氯化镨对小鼠骨髓细胞微核率和核异常率的影响及硒的干预作用[J].环境与职业医学,2011,28(9):547-550,4.
