环境与职业医学2011，Vol.28Issue(9)：577-581,5.
Aroclor1254对斑马鱼胚胎发育、肝脏组织结构及其卵黄蛋白原基因的影响
Effects of Aroclor1254 on Embryo Development,Histology and Vitellogenin Expression in Liver of Zebrafish
摘要
Abstract
[Objective] To evaluating the effect of Aroclorl254 on embryo development, histology and vitellogenin gene expression in zebrafish liver so as to provide basicdata of toxic effects of Aroclor1254 and to select sensitive markers for early detection and warning using zebrafish. [ Methods ] Zebrafish and its embryos were exposed to different doses of Aroclor1254. Embryo development technique, histological examination and real-time quantitative polymerase chain reaction (RT-PCR) were used to detect the effect of embryonic development, morphological changes and vitellogenin (vtg) gene expression. [ Results ] The zebrafish embryos test showed that Aroclorl254 induced liver degeneration and hypoplasia at the doses of 3.3, 6.5, 10.0mg/L. The embryo exhibited part of yolk sac degeneration at 3.3 mg/L, but most degeneration at 6.5, 10.0 mg/L. The histological examination showed that the morphological changes such as cell swelling, cytoplasm loose, vacuoles increase and lipid deposition were observed in liver at doses of 2.0, 10.0, 50 μg/L. The result of real-time quantitative RT-PCR revealed that a significant down-regulation of mRNA vtg gene expression in female zebrafish as compared to the control group, especially at dose of 2.0μg/L. However, the mRNA vtg gene expression in the male zebrafish was significantly higher than that in the control group at dose of 2.0(μg/L. [ Conclusion ] Aroclor1254 has toxic effects on zebrafish and its embryo. Liver could be one of the most important toxic target organs.关键词
Aroclor1254/斑马鱼/肝脏/卵黄蛋白原Key words
Aroclor1254/ zebrafish/ liver/ vitellogenin分类
医药卫生引用本文复制引用
徐娟,吴南翔,陈琼姜,宋扬,陈汝家..Aroclor1254对斑马鱼胚胎发育、肝脏组织结构及其卵黄蛋白原基因的影响[J].环境与职业医学,2011,28(9):577-581,5.基金项目
卫生部科研基金(编号:WHJ2008-2-015) （编号:WHJ2008-2-015）
