江苏大学学报（医学版）2011，Vol.21Issue(5)：375-377,381,4.
鼠白介素28重组腺病毒感染小鼠后的表达
The expression of mil-28 gene in the rat transfected with rat interleukin 28 recombinant adenovirus vector
摘要
Abstract
Objective: To 9tudy the mil-28 expression in the rat transfected with the interleukin 28 re-combinant adenovirus vector. Methods: Transfect the recombinant mil-28 adenovirus vector into the rat , detect the mil-28 gene expression with real-time PCR and Western blot. Results: The interleukin 28 recombinant adenovirus vector in the rat can express stably , and at the same time the rat can grow normally. In the muscle , the Ad-mlFN-lambda 2 group express the mlFN-lambda 2 gene at a higher level , while the blank group and the LacZ group at a lower level by the real-time PCR; Detected with the Western blot , Ad-mlFN-lambda 2 group appeared obviously about mlFN-lambda 2 protein strip , while the blank group and the LacZ group with lighter bands. Conclusion: The stable expression of the mIFN -lambda 2 gene in the rat was detected after transfected with the interleukin 28 recombinant adenovirus vector , and the rat can grow normally , which give a guidance to the vivo biological activity analysis of the mlFN-lambda 2 in the future.关键词
mIFN-λ2/感染/基因表达Key words
IFN-A2/ transfection/ gene expression分类
医药卫生引用本文复制引用
张金,朱涛峰,刘洋,丁浩,步雪峰,严玉兰..鼠白介素28重组腺病毒感染小鼠后的表达[J].江苏大学学报（医学版）,2011,21(5):375-377,381,4.基金项目
镇江市社会发展项目(SH2010036) （SH2010036）
