军事医学2011，Vol.35Issue(10)：746-748,753,4.
霍乱弧菌外膜蛋白W的原核表达及抗原性分析
Expression of Vibrio cholerae outer membrane protein W and identification of its antigenicity
摘要
Abstract
Objective To obtain Vibrio cholerae outer membrane protein W expressed in E. Coli ( DE3 ) in order to produce its polyclonal antibody. Methods The ompW gene amplified from V. Cholerae by PCR was inserted into expression plasmid pET-32a( + ) to construct recombinant plasmid pET-32a( + )-ompW that was transformed into E. Coli BL21 ( DE3 ) with IPTG induction for the expression OmpW. The protein OmpW was purified with degeneration and renaturation and used to immunize rabbits to prepare anti-OmpW antibody,the property of which was identified. Results The ompW gene was obtained and the prokaryotic expression system constructed. The positive recombinant clone was identified by restriction enzyme digestion analysis and DNA sequencing. Induced with IPTG, OmpW was expressed in E. Coli BL2l( DE3 ). The purified recombinant protein was used to immunize rabbits. It could irritate the production of specific antibody. Conclusion The recombinant expression plasmid is constructed successfully and expressed in E. Coli. The anti-OmpW antibody has a high titer, which can be used to detect V. Cholerae in the future.关键词
外膜蛋白W/霍乱弧菌/原核表达/抗原性Key words
outer membrane protein W/ Vibrio cholerae/ prokaryotic expression/ antigenicity分类
医药卫生引用本文复制引用
李建华,王忠田,何君,左庭婷,韩雪莲,李岩伟,朱虹,端青..霍乱弧菌外膜蛋白W的原核表达及抗原性分析[J].军事医学,2011,35(10):746-748,753,4.基金项目
"艾滋病和病毒性肝炎等重大传染病防治"科技重大专项(2008ZX10004-003) （2008ZX10004-003）
