生物信息学2011，Vol.9Issue(3)：189-191,3.DOI:10.3969/j.issn.1672-5565.2011.03.003
利用siRNA高通量测序技术筛查蚊子体内携带的RNA病毒
Screening for RNA Viruses in Mosquitoes by High-throughput Sequencing of siRNA
摘要
Abstract
Insects obtains immunity against RNA viruses through small interference RNA (siRNA) mechanism. Here we adopt Next - generation sequencing technique to perform high - throughput sequencing of mosquito siRNA and then screening for RNA virus sequences with bioinformatics methods. We found that the Aedes albopictus and Culex quinquefasciatus captured in Yunnan, China contains the genonomic information of Marayo virus, Omsk hemorrhagic fever virus and Ilheus virus. This research established a new strategy to find naturally harbored RNA viruses in mosquitoes, and this strategy could be applied to virus surveillance in vector insects.关键词
高通量测序/小干扰RNA/病毒,白纹伊蚊/致倦库蚊Key words
high-throughput sequencing/small interference RNA/virus/Aedes albopictus/Culex quinquefasciatus分类
生物科学引用本文复制引用
陈斌,何后军,陈禹保,童贻刚,刘玮,龚正达,张丽云,朱大强,李存,江晓芳,安小平,米志强,范华昊..利用siRNA高通量测序技术筛查蚊子体内携带的RNA病毒[J].生物信息学,2011,9(3):189-191,3.基金项目
国家自然科学基金(编号30460124,30660160)和病原微生物生物安全国家重点实验室课题(编号:PBS2009A-04). （编号30460124,30660160）
