长春中医药大学学报Issue(5)：713-715,3.
重组免疫毒素EGF-TCSredlk治疗实体肿瘤实验研究
Antitumor Effect of Recombinant Immunotoxin EGF- TCSredlk on Tumor- bearing Mouse Model
摘要
Abstract
Objective:To further observe the anti - tumor effect of recombinant toxin, we carried tumor inhibition test in vivo in naked mouse tumor model. Methods: The hepatoma cell BEL - 7402 was inoculated subcutaneously on right armpit in naked mice and EGF - TCSredlk was injected intravenously (100 ,50 ,25 μg/kg) to mice for therapy after 6 days of inoclu-ation.There was control group and mice were killed after 2 days of drug withdrawal,and the tumors were weighed and the tumor inhibitory rate was calculated; Tumor tissues were examined by immunohistochemistry. Result: The illustrated that EGF - TCSredlk could inhibit tumor growth markedly.The inhibition rate was75.5% 、54.5%and 35.4% for high dosage group, middle dosage group and low dosage group respectively. Variance analysis illustrated that EGF - TCSredlk could markedly inhibit the growth of naked mouse tumors, variance analysis had significant difference (Welch = 82.617, P = 0.000); Immunohistochemistry experiment showed that EGF - TCSredlk could makedly inhibit tumor angiogenesis. There were no visible blood vessels in tumor tissues in high dosage group and there were fewer blood vessels in tumor tissues in middle and low dosage group than those in control group, which revealed that perhaps EGF - TCSredlk inhibited tumor growth and migration by inhibiting tumor angiogenesis. Conclusions: EGF - TCSredlk can inhibit the growth of solid tumors in tumor - bearing mice and the priliminary results suggested the potential applications of this preparation in cancer therapy.关键词
天花粉蛋白/表皮生长因子/免疫毒素/靶向治疗/小鼠Key words
trichosanthin/ epidermal growth factor/ immunotoxin/ targeted therapy分类
医药卫生引用本文复制引用
李咏梅,霍云华,杨明会..重组免疫毒素EGF-TCSredlk治疗实体肿瘤实验研究[J].长春中医药大学学报,2011,(5):713-715,3.基金项目
中国博士后基金资助项目(基金编号:20090450)。 （基金编号:20090450）
