现代妇产科进展2011，Vol.20Issue(7)：555-558,4.
低氧通过HIF-1α和HIF-2α上调SKOV3细胞Syndecan-1的表达
Hypoxia dependent HIF-1α and HIF-2α up regulates the expression of Syndecan-1 in SKOV3 cells
辛卫娟 1李婷 2赵鑫 2丁岩 1吴乾渝2
作者信息
- 1. 复旦大学附属妇产科医院妇产科，上海200032
- 2. 同济大学附属东方医院妇产科
- 折叠
摘要
Abstract
Objective: To investigate the expression of hypoxia inducible factor-la ( HIF-lα), hypoxia inducible factor-2a ( HIF-2a ) , and Syndecans-1, and their relationship during hypoxia in human ovarian cancer cell line SKOV3. Methods: The transcription factor binding sites of Syndecan-1 were analyzed with Matinspector tools. In addition, under hypoxic environments and an exposure to Rapamycin,a suppressor for hypoxia inducible factors,SKOV3 cells were subject to fluorescent quantitative RT-PCR for HIF-lα,HIF-2a and Syndecan-1 mR-NA expressions,Western blot was made for HIF-la and HIF-2a proteins expressions. Results: There were three HIFF( hvpoxia-inducible factor family) /HIF conserved sequences in the Syn-decan-1 transcription factor binding sites. Under the condition of hypoxia, the expressions of HIF-lα mRNA reduced, the expressions of HIF-lα protein, HIF-2α mRNA and protein increased (P<0.05). Rapamycin couldn't inhibit the expression of HIF-la protein,but could inhibit the expression of HIF-2a. The change of Syndecan-1 mRNA was consistent with that of the HIF-la and HIF-2a proteins. Conclusion: Hypoxia can induce expression of HIF-la and HIF-2a and promote the expression of Syndecan-1 through the HIF-la and HIF-2a pathway.关键词
卵巢肿瘤；Syndecan-1；雷帕霉素；HIF-1α；HIF-2αKey words
Ovarian neoplasms/Syndecan-1/Rapamycin/HIF-lα/HIF-2α分类
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辛卫娟,李婷,赵鑫,丁岩,吴乾渝..低氧通过HIF-1α和HIF-2α上调SKOV3细胞Syndecan-1的表达[J].现代妇产科进展,2011,20(7):555-558,4.
